• Immunocytochemistry staining of HeLa cells fixed with-20℃ Methanol and using HP1-gamma (200137-5G10, dilution 1:200) mouse mAb (green) and Histone H3.1 (Phospho-Ser10) (310045, dilution 1:200) Rabbit pAb (red). DAPI was used to stain nucleus (blue).
  • Immunohistochemical analysis of paraffin-embedded Prostate Cancer using HP1-gamma mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
  • Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-HP1-gamma mouse mAb (dilution 1:200).
  • Immunoprecipitation analysis of Hela cell lysates using HP1-gamma mouse mAb.
  • Western blot detection of HP1-gamma in Hela, 3T3, C6, COS7 and CHO-K1 cell lysates using HP1-gamma mouse mAb (1:1000 diluted).Predicted band size:22KDa.Observed band size:22KDa.

Anti-CBX3 antibody [5G10-F7-A12] (STJ99038)

SKU:
STJ99038

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IF/IP/IHC
Reactivity: Mouse/Human/Monkey/Hamster/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Chromobox protein homolog 3 is suitable for use in Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 5G10-F7-A12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX3
Gene ID: 11335
Uniprot ID: CBX3_HUMAN
Specificity: This antibody detects endogenous levels of HP1-gamma and does not cross-react with related proteins.
Immunogen: Purified recombinant human HP1-gamma protein fragments expressed in E.coli.
Function Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs).
Protein Name Chromobox Protein Homolog 3
Hech
Heterochromatin Protein 1 Homolog Gamma
Hp1 Gamma
Modifier 2 Protein
Database Links Reactome: R-HSA-427389
Reactome: R-HSA-73772
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Associates With Euchromatin And Is Largely Excluded From Constitutive Heterochromatin
May Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential)
Alternative Antibody Names Anti-Chromobox Protein Homolog 3 antibody
Anti-Hech antibody
Anti-Heterochromatin Protein 1 Homolog Gamma antibody
Anti-Hp1 Gamma antibody
Anti-Modifier 2 Protein antibody
Anti-CBX3 antibody

Information sourced from Uniprot.org

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