Anti-CBX3 antibody (1-100) [S1MR] (STJ11102031)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal HP1 gamma (1-100) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S1MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CBX3 |
| Gene ID | 11335 |
| Uniprot ID | CBX3_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MASNKTTLQKMGKKQNGKSK KVEEAEPEEFVVEKVLDRRV VNGKVEYFLKWKGFTDADNT WEPEENLDCPELIEAFLNSQ KAGKEKDGTKRKSLSDSESD |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HP1 gamma/CBX3 (NP_057671.2). |
Additional Info
| Post Translational Modifications | Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. |
| Function | Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1. Mediates the recruitment of NIPBL to sites of DNA damage at double-strand breaks (DSBs). |
| Protein Name | Chromobox Protein Homolog 3HechHeterochromatin Protein 1 Homolog GammaHp1 GammaModifier 2 Protein |
| Database Links | Reactome: R-HSA-427389Reactome: R-HSA-73772Reactome: R-HSA-8953750 |
| Cellular Localisation | NucleusAssociates With Euchromatin And Is Largely Excluded From Constitutive HeterochromatinMay Be Associated With Microtubules And Mitotic Poles During Mitosis (Potential) |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 3 antibodyAnti-Hech antibodyAnti-Heterochromatin Protein 1 Homolog Gamma antibodyAnti-Hp1 Gamma antibodyAnti-Modifier 2 Protein antibodyAnti-CBX3 antibody |
Information sourced from Uniprot.org