Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of various lysates using ASS1 Rabbit mAb (STJ11102420) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-ASS1 antibody (1-100) [S0MR] (STJ11102420)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using ASS1 Rabbit polyclonal antibody (STJ117795) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ASS1 antibody (1-412) (STJ117795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (STJ11106419) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Asymmetric DiMethyl-Histone H3-R17 antibody (STJ11106419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-ATAD2B IP antibody [SAA0243] (STJA0018758)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP/IP-MS

Reactivity :

Human

£378.50 - £1,632.50

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Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B antibody (STJ11104924) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATAD3A/ATAD3B antibody (1-240) (STJ11104924)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-ATAD5/FRAG1 antibody (Internal) (STJ71103)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ATF1 antibody (176-225 aa) (STJ91744)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of COLO cells using ATF-1 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ATF1 antibody (31-80 aa) (STJ91743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using ATF-2 Polyclonal Antibody

Anti-ATF2 antibody (29-78 aa) (STJ91746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-ATF2 antibody (35-84 aa) (STJ91750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ATF2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ATF2 antibody (38-87 aa) (STJ91751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Anti-ATF2 antibody (40-89 aa) (STJ91745)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa and 293 cells, using ATF-2 Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-ATF2 antibody (441-490 aa) (STJ91749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells, using ATF2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ATF2 antibody (456-505 aa) (STJ91748)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat

£48.00 - £252.00

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Anti-ATF2 antibody (79-128 aa) (STJ91747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using ATF-2 monoclonal antibody against NIH/3T3 cell lysate.

Anti-ATF2 antibody [4F12] (STJ97854)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£144.00 - £348.00

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Western blot analysis of 293T-UV cells using ATF-3 Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-ATF3 antibody (131-180 aa) (STJ91752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from HeLa cells, using ATF4 Rabbit polyclonal antibody (STJ22704) at 1:1000 dilution. HeLa cells were treated by tunicamycin (2 Mu g/mL) for 8 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-ATF4 antibody (1-351) (STJ22704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of Hep G2 cells using ATF4 Rabbit polyclonal antibody (STJ11101481) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF4 antibody (260-351) (STJ11101481)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using ATF5 Rabbit polyclonal antibody (STJ11100125) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATF5 antibody (101-282) (STJ11100125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using ATF-5 Polyclonal Antibody diluted at 1：1000

Anti-ATF5 antibody (221-270 aa) (STJ91753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ22705) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (190-670) (STJ22705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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EB012080 (1µg/ml) staining of HeLa (A) and MCF7 (B) cell lysate, and HeLa (C) and Jurkat (D) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ATF6 antibody (437-449) (STJ72994)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Rat/Dog/Pig/Cow

£312.50

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Immunofluorescence analysis of PC-12 cells using ATF6 Rabbit polyclonal antibody (STJ11103832) at a dilution of 1:200 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-ATF6 antibody (600-670) (STJ11103832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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STJ73653 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-ATF6 antibody (C or N-Term) (STJ73653)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of various cells using ATF-6 Beta Polyclonal Antibody

Anti-ATF6B antibody (401-450 aa) (STJ91754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of various cells using ATF-7 Polyclonal Antibody

Anti-ATF7 antibody (41-90 aa) (STJ91755)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ATF7 antibody [SAA2118] (STJA0022319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Anti-ATF7 antibody [SAA2119] (STJA0022320)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IF/IP

Reactivity :

Human

£378.50 - £1,632.50

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Immunohistochemical analysis of paraffin-embedded human-tonsil, antibody was diluted at 1:200

Anti-ATG10 antibody (1-50 aa) (STJ98826)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of NIH-3T3 cells using ATG10 Rabbit monoclonal antibody (STJ11102331) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG10 antibody (121-220) [S1MR] (STJ11102331)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Immunofluorescence analysis of U2OS cells using ATG12 Rabbit polyclonal antibody (STJ118058) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG12 antibody (1-100) (STJ118058)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-Atg12 antibody at 1:500 dilution; 293HEK cells transfected with GFP-Atg12; lysate at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-ATG12 antibody (65aa N-Term) (STJ140014)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis of various lysates, using ATG13 antibody (STJ22706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 25s.

Anti-ATG13 antibody (281-517) (STJ22706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ATG13 antibody (STJ197439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ATG13 antibody (STJA0006706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using ATG14 Rabbit polyclonal antibody (STJ29664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG14 antibody (340-440) (STJ29664)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human small intestinal carcinoma tissue. 1, primary Antibody was diluted at 1:200 (4°C overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200

Anti-ATG14 antibody (STJ197438)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using ATG16L1 Rabbit polyclonal antibody (STJ113881) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (200-300) (STJ113881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of A-549 cells using ATG16L1 antibody (STJ22707). Blue: DAPI for nuclear staining.

Anti-ATG16L1 antibody (90-300) (STJ22707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit polyclonal antibody (STJ28372) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG3 antibody (1-314) (STJ28372)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunocytochemistry analysis of ATG3 (green) in Hela using ATG3 antibody, and DAPI (blue)

Anti-ATG3 antibody (2-20 aa) [R06-9E7] (STJA0010998)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

£246.50

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Immunofluorescence analysis of NIH/3T3 cells using ATG4A Rabbit polyclonal antibody (STJ22708) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ATG4A antibody (210-398) (STJ22708)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Colo using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG4A antibody (81-130 aa) (STJ91758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-ATG4A antibody (STJ197437)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Jurkat cells using Atg4b Polyclonal Antibody

Anti-ATG4B antibody (71-120 aa) (STJ91759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-ATG4B antibody (STJ197436)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-ATG4C antibody (21-70 aa) (STJ91760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Monkey

£48.00 - £252.00

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