• Immunofluorescence analysis of U-251MG cells using AP2M1 Rabbit monoclonal antibody (STJ11101275) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using AP2M1 Rabbit monoclonal antibody (STJ11101275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AP2M1 antibody (300-400) [S5MR] (STJ11101275)

SKU:
STJ11101275

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AP2M1 (300-400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AP2M1
Gene ID: 1173
Uniprot ID: AP2M1_HUMAN
Immunogen Region: 300-400
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 300-400 of human AP2M1 (Q96CW1).
Immunogen Sequence: KLEVKVVIKSNFKPSLLAQK IEVRIPTPLNTSGVQVICMK GKAKYKASENAIVWKIKRMA GMKESQISAEIELLPTNDKK KWARPPISMNFEVPFAPSGL K
Tissue Specificity Expressed in the brain (at protein level).
Post Translational Modifications Phosphorylation at Thr-156 increases the affinity of the AP-2 complex for cargo membrane proteins during the initial stages of endocytosis.
Function Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10. The AP-2 mu subunit binds to transmembrane cargo proteins.it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1. Plays a role in endocytosis of frizzled family members upon Wnt signaling.
Protein Name Ap-2 Complex Subunit Mu
Ap-2 Mu Chain
Adaptin-Mu2
Adaptor Protein Complex Ap-2 Subunit Mu
Adaptor-Related Protein Complex 2 Subunit Mu
Clathrin Assembly Protein Complex 2 Mu Medium Chain
Clathrin Coat Assembly Protein Ap50
Clathrin Coat-Associated Protein Ap50
Ha2 50 Kda Subunit
Plasma Membrane Adaptor Ap-2 50 Kda Protein
Database Links Reactome: R-HSA-164939
Reactome: R-HSA-167590
Reactome: R-HSA-177504
Reactome: R-HSA-182218
Reactome: R-HSA-190873
Reactome: R-HSA-196025
Reactome: R-HSA-2132295
Reactome: R-HSA-3928665
Reactome: R-HSA-416993 Q96CW1-2
Reactome: R-HSA-437239
Reactome: R-HSA-5099900
Reactome: R-HSA-5140745
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8866427
Reactome: R-HSA-8964038
Reactome: R-HSA-9679191
Cellular Localisation Cell Membrane
Membrane
Coated Pit
Peripheral Membrane Protein
Cytoplasmic Side
Ap-2 Appears To Be Excluded From Internalizing Ccvs And To Disengage From Sites Of Endocytosis Seconds Before Internalization Of The Nascent Ccv
Alternative Antibody Names Anti-Ap-2 Complex Subunit Mu antibody
Anti-Ap-2 Mu Chain antibody
Anti-Adaptin-Mu2 antibody
Anti-Adaptor Protein Complex Ap-2 Subunit Mu antibody
Anti-Adaptor-Related Protein Complex 2 Subunit Mu antibody
Anti-Clathrin Assembly Protein Complex 2 Mu Medium Chain antibody
Anti-Clathrin Coat Assembly Protein Ap50 antibody
Anti-Clathrin Coat-Associated Protein Ap50 antibody
Anti-Ha2 50 Kda Subunit antibody
Anti-Plasma Membrane Adaptor Ap-2 50 Kda Protein antibody
Anti-AP2M1 antibody
Anti-CLAPM1 antibody
Anti-KIAA0109 antibody

Information sourced from Uniprot.org

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