• Immunofluorescence analysis of C6 cells using APOBEC3G Rabbit polyclonal antibody (STJ115559) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using APOBEC3G Rabbit polyclonal antibody (STJ115559) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using APOBEC3G Rabbit polyclonal antibody (STJ115559) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using APOBEC3G Rabbit polyclonal antibody (STJ115559) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of PC-3 cells, using APOBEC3G antibody (STJ115559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-APOBEC3G antibody (244-257) (STJ115559)

SKU:
STJ115559

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOBEC3G (244-257) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC3G
Gene ID: 60489
Uniprot ID: ABC3G_HUMAN
Immunogen Region: 244-257
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 244-257 of human APOBEC3G (NP_068594.1).
Immunogen Sequence: NQAPHKHGFLEGRH
Tissue Specificity Expressed in spleen, testes, ovary and peripheral blood leukocytes and CD4+ lymphocytes. Also expressed in non-permissive peripheral blood mononuclear cells, and several tumor cell lines.no expression detected in permissive lymphoid and non-lymphoid cell lines. Exists only in the LMM form in peripheral blood-derived resting CD4 T-cells and monocytes, both of which are refractory to HIV-1 infection. LMM is converted to a HMM complex when resting CD4 T-cells are activated or when monocytes are induced to differentiate into macrophages. This change correlates with increased susceptibility of these cells to HIV-1 infection.
Post Translational Modifications Ubiquitinated in the presence of HIV-1 Vif. Association with Vif targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway. Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity.
Function DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons.
Protein Name Dna Dc->Du-Editing Enzyme Apobec-3g
Apobec-Related Cytidine Deaminase
Apobec-Related Protein
Arcd
Apobec-Related Protein 9
Arp-9
Cem-15
Cem15
Deoxycytidine Deaminase
A3g
Database Links Reactome: R-HSA-180585
Reactome: R-HSA-180689
Cellular Localisation Cytoplasm
Nucleus
P-Body
Mainly Cytoplasmic
Small Amount Are Found In The Nucleus
During Hiv-1 Infection
Virion-Encapsidated In Absence Of Hiv-1 Vif
Alternative Antibody Names Anti-Dna Dc->Du-Editing Enzyme Apobec-3g antibody
Anti-Apobec-Related Cytidine Deaminase antibody
Anti-Apobec-Related Protein antibody
Anti-Arcd antibody
Anti-Apobec-Related Protein 9 antibody
Anti-Arp-9 antibody
Anti-Cem-15 antibody
Anti-Cem15 antibody
Anti-Deoxycytidine Deaminase antibody
Anti-A3g antibody
Anti-APOBEC3G antibody
Anti-MDS019 antibody

Information sourced from Uniprot.org

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