• Immunoprecipitation analysis of 200 Mu g extracts of K-562 cells, using 3 Mu g APOBEC3C antibody (STJ116721). Western blot was performed from the immunoprecipitate using APOBEC3C antibody (STJ116721) at a dilution of 1:1000.
  • Western blot analysis of lysates from K-562 cells, using APOBEC3C Rabbit polyclonal antibody (STJ116721) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-APOBEC3C antibody (1-190) (STJ116721)

SKU:
STJ116721

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-APOBEC3C (1-190) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: APOBEC3C
Gene ID: 27350
Uniprot ID: ABC3C_HUMAN
Immunogen Region: 1-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human APOBEC3C (NP_055323.2).
Immunogen Sequence: MNPQIRNPMKAMYPGTFYFQ FKNLWEANDRNETWLCFTVE GIKRRSVVSWKTGVFRNQVD SETHCHAERCFLSWFCDDIL SPNTKYQVTWYTSWSPCPDC AGEVAEFLARHSNVNLTIFT ARLYYFQYPCYQEGLRSLSQ EGVAVEIMDYEDFKYCWENF VYNDNEPFKPWKGLKTNFRL LKRRLRESLQ
Tissue Specificity Expressed in spleen, testes, peripherical blood lymphocytes, heart, thymus, prostate and ovary.
Function DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity against simian immunodeficiency virus (SIV), hepatitis B virus (HBV), herpes simplex virus 1 (HHV-1) and Epstein-Barr virus (EBV) and may inhibit the mobility of LTR and non-LTR retrotransposons. May also play a role in the epigenetic regulation of gene expression through the process of active DNA demethylation.
Protein Name Dna Dc->Du-Editing Enzyme Apobec-3c
A3c
Apobec1-Like
Phorbolin I
Database Links Reactome: R-HSA-72200
Reactome: R-HSA-75094
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Dna Dc->Du-Editing Enzyme Apobec-3c antibody
Anti-A3c antibody
Anti-Apobec1-Like antibody
Anti-Phorbolin I antibody
Anti-APOBEC3C antibody
Anti-APOBEC1L antibody
Anti-PBI antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance