Immunofluorescence Antibodies | St John's Laboratory

STJ70605 (0. 3µg/ml) staining of nuclear HeLa cell lysate (A) + peptide (B) and nuclear K562 cell lysate (C) + peptide (D). (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-RXR alpha antibody (Internal) (STJ70605)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat

£312.50

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Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR Alpha antibody (STJ11101642) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-RXRA antibody (1-100) [S2MR] (STJ11101642)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Anti-RXRA antibody [PT1300R] (STJA0040111)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Western blot analysis of various cells using RXR Gamma Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-RXRG antibody (171-220 aa) (STJ95560)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Western blot analysis of HepG2-UVmouse brainAD293 PC-3 cells using RyR-2 Polyclonal Antibody diluted at 1：2000

Anti-RYR2 antibody (2774-2823 aa) (STJ95561)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of paraffin-embedded mouse heart using RYR2 Rabbit polyclonal antibody (STJ25428) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RYR2 antibody (4932-4948) (STJ25428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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S100 alpha 6/PRA antibody western blot HeLa A549 STJA0037553

Anti-S100 alpha 6 antibody (STJA0037553)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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S100 alpha 6 antibody western blot 3T3 lysates STJA0035355

Anti-S100 alpha6 antibody [R01-8A4] (STJA0035355)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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S100 alpha 6 antibody western blot HeLa STJA0036266

Anti-S100 alpha6 antibody [R07-1F6] (STJA0036266)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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S100A9 antibody western blot human tonsil lysates STJA0036253

Anti-S100 alpha9 antibody [R02-7C5] (STJA0036253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human

£133.50 - £281.50

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S100A9 antibody western blot mouse spleen lung STJA0034836

Anti-S100 alpha9 antibody [R05-3C3] (STJA0034836)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Mouse

£133.50 - £281.50

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Anti-S100 beta antibody [SM5059] (STJA0005059)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Immunohistochemistry of paraffin embedded human peripheral nerve sheath tumors using S100B at dilution of 1:1500

Anti-S100 beta antibody [SM8239] (STJA0018239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-S100/S100A1 antibody [SRM14600] (STJ11109330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of various cells using S-100 Alpha Polyclonal Antibody

Anti-S100A1 antibody (10-59 aa) (STJ95562)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) 293T, 2) Mouse Brain Tissue with S100 Rabbit pAb diluted at 1:2,000.

Anti-S100A1 antibody (STJ197337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis using S-100 Alpha monoclonal antibody against truncated S-100 Alpha recombinant protein.

Anti-S100A1 antibody [2C8B8/2C8E8/3C8D8] (STJ98373)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Western blot analysis of 293F, using S100A12 antibody (STJ27281) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-S100A12 antibody (1-92) (STJ27281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Confocal imaging of paraffin-embedded Human colon using FSP1/S100A4 Rabbit monoclonal antibody (STJ11101646, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-S100A4 antibody (2-101) [S6MR] (STJ11101646)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Western blot analysis of lysates from U-251 MG cells using S100A6 Rabbit pAb (STJ11106830) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-S100A6 antibody (1-90 aa) (STJ11106830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using [KO Validated] S100A6 Rabbit polyclonal antibody (STJ25432) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-S100A6 antibody (1-90) (STJ25432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Confocal imaging of C2C12 cells using S100A6 Rabbit monoclonal antibody (STJ11101874, dilution 1:400) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-S100A6 antibody (1-90) [S4MR] (STJ11101874)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Human malignant melanoma tissue was stained with Anti-S100A6 (ABT-S100A6) Antibody

Anti-S100A6 antibody (50-90 aa) [ABT-S100A6] (STJ197002)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Anti-S100A6 antibody [SM5011] (STJA0005011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£228.50

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Confocal immunofluorescence analysis of U-2 OS cells using S100A8 Polyclonal Antibody (STJ117510) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ117510)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded human skin cancer using S100A8 Rabbit polyclonal antibody (STJ25434) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-S100A8 antibody (1-93) (STJ25434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ72798 (0. 03µg/ml) staining of Human Lung lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-S100A8 antibody (Internal) (STJ72798)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/IHC

Reactivity :

Human

£312.50

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Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/FC/ELISA/IF/IP/WB

Reactivity :

Human/Rhesus

£926.50

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Western blot analysis of lysates from Mouse spleen using S100A9 Rabbit pAb (STJ111884) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-S100A9 antibody (20-114) (STJ111884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ71826 (1µg/ml) staining of Human Bone Marrow (A) and (0. 5µg/ml) Gastrointestinal cancer (B) lysate and negative control HepG2 (C) lysate. 5µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-S100A9 antibody (Internal) (STJ71826)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human

£312.50

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Anti-S100A9 antibody [SRM13519] (STJ11108249)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse

£126.50 - £554.50

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Human Kidney tissue was stained with Anti-S100 (ABT-S100B) Antibody

Anti-S100B antibody (1-50 aa) [ABT-S100B] (STJ197003)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£180.00 - £261.00

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g S100B antibody (STJ29811). Western blot was performed from the immunoprecipitate using S100B antibody (STJ29811) at a dilution of 1:500.

Anti-S100B antibody (1-92) (STJ29811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of Rat brain, using S100B rabbit monoclonal antibody (STJ11101645) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-S100B antibody (1-92) [S5MR] (STJ11101645)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£143.50 - £618.50

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Fluorescence multiplex immunohistochemical analysis of mouse brain tissue (formalin-fixed paraffin-embedded section). The immunostaining was performed by Sextuple-Fluorescence kit. GFAP mouse monoclonal antibody (STJA0006242 green), S100 mouse monoclonal antibody (STJ197266 cyan), Neurofilament mouse monoclonal antibody (purple), Iba 1 mouse monoclonal antibody (red), CD8 a mouse monoclonal antibody (yellow), The section was incubated in 5 rounds of staining; sequentially for Anti-antibodies; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain. Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (Excilone).

Anti-S100B antibody (2-92 aa) [ABT094] (STJ197266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

£180.00 - £261.00

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Western blot analysis of 1) Mouse Brain Tissue, 2) Rat Brain Tissue with S100β Rabbit pAb diluted at 1:2,000.

Anti-S100B antibody (STJ197336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis using S-100 Beta monoclonal antibody against full-length S-100 Beta recombinant protein.

Anti-S100B antibody [9A11B9] (STJ98374)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-S100B antibody [PT0461R] (STJA0039450)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£285.00 - £540.00

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Western blot analysis of COLO cells using S-100Z Polyclonal Antibody

Anti-S100Z antibody (2-51 aa) (STJ95569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using EDG-1 Polyclonal Antibody

Anti-S1PR1 antibody (206-255 aa) (STJ92824)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of COLO205 cells using EDG-1 Polyclonal Antibody

Anti-S1PR1 antibody (5-54 aa) (STJ92825)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of various cells using EDG-5 Polyclonal Antibody

Anti-S1PR2 antibody (261-310 aa) (STJ92832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-S6 Ribosomal Protein (RPS6) antibody [SRM13224] (STJ11107954)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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S6K1 antibody western blot 293T STJA0035716

Anti-S6K1 antibody [R07-7V3] (STJA0035716)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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S6K2 antibody western blot K562 lysates STJA0036854

Anti-S6K2 antibody [R06-5O2] (STJA0036854)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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SA2 antibody western blot K562 Raw2647 lysates STJA0035474

Anti-SA2 antibody [R14-3R-6] (STJA0035474)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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STJ70427 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SAE2/UBA2 antibody (N-Term) (STJ70427)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF

Reactivity :

Human/Mouse/Cow

£312.50

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Western blot analysis of PC12 cells using SAMHD1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SAMHD1 antibody (431-480 aa) (STJ96856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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