STJ70427 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-SAE2/UBA2 antibody (N-Term) (STJ70427)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ70427-100
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General Information

Short DescriptionGoat polyclonal antibody anti-SAE2/UBA2 (N-Term) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
ApplicationsPep-ELISA/WB/IF
HostGoat
ReactivityHuman/Mouse/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:16000.
WB: This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013;77 (7) :1575-8. PMID: 23832333.
IF: Strong expression of th
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolUBA2
Gene ID10054
Uniprot IDSAE2_HUMAN
Accession NumberNP_005490
Immunogen RegionN-Term
Immunogen SequenceALSRGLPRELAEA

Additional Info

Function The heterodimer acts as an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.
Protein Name Sumo-Activating Enzyme Subunit 2
Anthracycline-Associated Resistance Arx
Ubiquitin-Like 1-Activating Enzyme E1b
Ubiquitin-Like Modifier-Activating Enzyme 2
Database Links Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Cellular Localisation Cytoplasm
Nucleus
Shuttles Between The Cytoplasm And The Nucleus
Sumoylation Is Required Either For Nuclear Translocation Or Nuclear Retention
Alternative Antibody Names Anti-Sumo-Activating Enzyme Subunit 2 antibody
Anti-Anthracycline-Associated Resistance Arx antibody
Anti-Ubiquitin-Like 1-Activating Enzyme E1b antibody
Anti-Ubiquitin-Like Modifier-Activating Enzyme 2 antibody
Anti-UBA2 antibody
Anti-SAE2 antibody
Anti-UBLE1B antibody
Anti-HRIHFB2115 antibody

Information sourced from Uniprot.org

Citations

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