![Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/149019/346630/STJ16100301_1__27068.1681998701.jpg?c=1 "Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)")
![Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/149019/346630/STJ16100301_1__27068.1681998701.jpg?c=1 "Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)")
Anti-S100A8/A9 antibody [2+70E+11] (STJ16100301)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
General Information
| Short Description | Mouse monoclonal anti-S100A8/A9 for use in IHC-F, FC, ELISA, IF, IP and WB in Human and Rhesus samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F/FC/ELISA/IF/IP/WB |
| Host | Mouse |
| Reactivity | Human/Rhesus |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 2+70E+11 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Concentration | 100 Mu g/mL |
| Dilution Range | FC 1:50WB 1:50IHC 1:50IP 1:50 |
| Formulation | PBS with 0.1% BSA and 0.02% sodium azide |
| Storage Instruction | Store at 2-8°C for up to 1-year, upon receipt. |
Target Information
Additional Info
| Background | The monoclonal antibody 27E10 recognizes an epitope specific for the S100A8/A9 heterocomplex that is not exposed on the individual subunits S100A8 (MRP8, calgranulin-A) or S100A9 (MRP14, calgranulin-B). The calcium-binding migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The expression of these proteins is largely confined to the cytosol of neutrophils and monocytes. The complex formation of these proteins is a calcium-dependent process. The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra-and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. The monoclonal antibody 27E10 can be used for early detection of inflammatory macrophages, for the characterization of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody 27E10 does not react with lymphocytes or platelets. |
Information sourced from Uniprot.org