Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of Neuro-2a cells, using Nestin antibody (STJ113858) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-NES antibody (1392-1621) (STJ113858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot of neonatal rat brain lysate showing specific immunolabeling of the ~220-240 kDa nestin doublet.

Anti-NES antibody [4D11] (STJA0003696)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat

$627.21

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Immunofluorescence analysis of NIH-3T3 cells using Nesprin 1 Rabbit monoclonal antibody (STJ11103130) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Nesprin 1 antibody (8200-8400) [S0MR] (STJ11103130)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Immunofluorescence analysis of NIH/3T3 cells using Nesprin 1 Rabbit polyclonal antibody (STJ11103244) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Nesprin 1 antibody (8220-8410) (STJ11103244)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-Nestin antibody [SRM15064] (STJ11109794)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human

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Immunofluorescence analysis of HeLa cells using NET1 antibody (STJ24743). Blue: DAPI for nuclear staining.

Anti-NET1 antibody (1-300) (STJ24743)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunofluorescence analysis of NIH/3T3 cells using NEU3 Rabbit polyclonal antibody (STJ115782) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEU3 antibody (222-461) (STJ115782)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Western blot analysis of various lysates using NeuN Mouse mAb (STJ11107255) at 1:5000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 90s.

Anti-NeuN antibody [SMM5346] (STJ11107255)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-Neurochondrin antibody (STJ501948)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$605.13

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Anti-Neurochondrin antibody {Biotin} (STJ501949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Immunohistochemistry of mouse tissue with Anti-Neurochondrin primary antibody (FITC) (STJ501950) at 1:50 dilution in buffer.

Anti-Neurochondrin antibody {FITC} (STJ501950)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$686.55

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Anti-Neurofilament antibody [NF-01] (STJ16100237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB/ICC

Reactivity :

Mammalian

$669.99

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Confocal imaging of paraffin-embedded Mouse brain tissue using Neurofilament L Rabbit monoclonal antibody (STJ11103250, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-Neurofilament L antibody (60-250) [S0MR] (STJ11103250)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$163.53 - $719.67

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Anti-Neuroligin-2 antibody [L107/24] (STJA0021207)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ICC/IHC/WB

Reactivity :

Human/Mouse/Rat

$293.25 - $1,634.61

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Western blot analysis of various lysates using Neutrophil Elastase (ELANE) Rabbit mAb (STJ11103639) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Neutrophil Elastase antibody (167-267) [S9MR] (STJ11103639)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

$163.53 - $719.67

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Immunofluorescence analysis of U-2 OS cells using NEXN Rabbit polyclonal antibody (STJ115102) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NEXN antibody (516-675) (STJ115102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g [KD Validated] NF2 Rabbit polyclonal antibody (STJ114897). Western blot was performed from the immunoprecipitate using NF2 antibody (STJ114897) at a dilution of 1:1000.

Anti-NF2 antibody (477-576) (STJ114897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.57 - $853.53

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Immunofluorescence analysis of Jurkat cells using NFAT2 Rabbit polyclonal antibody (STJ24751) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFATC1 antibody (20-300) (STJ24751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of rat spleen using NFATC2 Rabbit polyclonal antibody (STJ24752) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFATC2 antibody (1-300) (STJ24752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of various cell lines, using NFATC3 antibody (STJ28749) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-NFATC3 antibody (1-290) (STJ28749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of HeLa cells using NFE2L1 Rabbit polyclonal antibody (STJ116953) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NFE2L1 antibody (515-772) (STJ116953)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemistry analysis of NRF2 in paraffin-embedded human placenta using NRF2 Rabbit polyclonal antibody (STJ113550) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-NFE2L2 antibody (243-400) (STJ113550)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of lysates from NIH/3T3 cells using NRF2 Rabbit pAb (STJ113718) at 1:1000 dilution incubated overnight at 4 °C. NIH/3T3 cells were treated by MG132 (10 Mu M) at 37 °C for 90 minutes. <br/> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 30 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 45s.

Anti-NFE2L2 antibody (366-605) (STJ113718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of extracts of mouse brain, using NFIA antibody (STJ24756) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-NFIA antibody (180-330) (STJ24756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.

Anti-NFIC antibody [4E10-D8-F4] (STJ99075)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$205.62 - $496.80

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Immunofluorescence analysis of MCF-7 cells using NFIL3 antibody (STJ115964). Blue: DAPI for nuclear staining.

Anti-NFIL3 antibody (133-462) (STJ115964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

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Western blot analysis of mouse lung, using NFKB1 antibody (STJ28750) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-NFKB1 antibody (41-365) (STJ28750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of BALB-3T3 cells using NFKB1 Rabbit polyclonal antibody (STJ116954) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFKB1 antibody (41-968) (STJ116954)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunoprecipitation analysis of 150 Mu g extracts of MCF-7 cells using 3 Mu g NFKB1 antibody (STJ113719). Western blot was performed from the immunoprecipitate using NFKB1 antibody (STJ113719) at a dilution of 1:500.

Anti-NFKB1 antibody (740-964) (STJ113719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human

$152.49 - $697.59

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Immunofluorescence analysis of U2OS cells using NF-Kappa B2 antibody (STJ113721) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NFKB2 antibody (690-899) (STJ113721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$152.49 - $697.59

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Immunofluorescence analysis of U2OS cells using NF-Kappa B2 antibody (STJ24762) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-NFKB2 antibody (690-899) (STJ24762)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of HeLa cells using NFS1 antibody (STJ115347). Blue: DAPI for nuclear staining.

Anti-NFS1 antibody (208-457) (STJ115347)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of U-251 MG cells using NFS1 Rabbit polyclonal antibody (STJ28751) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NFS1 antibody (290-457) (STJ28751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Western blot analysis of various lysates using NGEF Rabbit polyclonal antibody (STJ118934) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-NGEF antibody (429-710) (STJ118934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NGEF antibody (C-Term) (STJA0005408)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$504.39

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Western blot analysis of extracts of various cell lines, using (STJ113724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NGFR antibody (273-427) (STJ113724)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Immunofluorescence analysis of MCF-7 cells using NGFRAP1 antibody (STJ29435).

Anti-NGFRAP1 antibody (1-111) (STJ29435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Western blot analysis of extracts of various cell lines, using NGLY1 antibody (STJ118309) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-NGLY1 antibody (1-300) (STJ118309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NGLY1 antibody [SRM14574] (STJ11109304)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Anti-NgR3 antibody [SRM13123] (STJ11107853)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of 293F-NHLH1, using NHLH1 rabbit polyclonal antibody (STJ118141) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NHLH1 antibody (1-60) (STJ118141)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

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Anti-NHLH1 antibody [SRM13424] (STJ11108154)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human

$0.00

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Anti-NHLRC1/Malin antibody [SRM14893] (STJ11109623)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$0.00

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Anti-Niemann Pick C1 antibody [SRM13618] (STJ11108348)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Western blot analysis of extracts of LNCaP cells, using NKX3. 1 antibody (STJ11103370) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-NKX3.1 antibody (1-80) (STJ11103370)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

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Immunofluorescence analysis of PC-12 cells using NLRC4 Rabbit polyclonal antibody (STJ11105310) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NLRC4 antibody (71-170) (STJ11105310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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Anti-NLRC4 antibody (85-170) (STJ29519)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

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Immunofluorescence analysis of SH-SY5Y cells using NLRP1 Rabbit polyclonal antibody (STJ118652) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-NLRP1 antibody (1370-1473) (STJ118652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$152.49 - $697.59

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