![Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87MG cells, using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176989/368132/STJ11104345_3__89946.1713133647.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and GPX4 knockdown (KD) U-87MG cells, using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of lysates from Jurkat cells, using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176989/368133/STJ11104345_4__07667.1713133648.jpg?c=1 "Western blot analysis of lysates from Jurkat cells, using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western blot analysis of various lysates using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/176989/368134/STJ11104345_5__14053.1713133648.jpg?c=1 "Western blot analysis of various lysates using [KD Validated] GPX4 Rabbit polyclonal antibody (STJ11104345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-GPX4 antibody (30-197) (STJ11104345)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Phospholipid Hydroperoxide Glutathione Peroxidase (30-197) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GPX4 |
| Gene ID | 2879 |
| Uniprot ID | GPX4_HUMAN |
| Immunogen Region | 30-197 |
| Immunogen Sequence | ASRDDWRCARSMHEFSAKDI DGHMVNLDKYRGFVCIVTNV ASQUGKTEVNYTQLVDLHAR YAECGLRILAFPCNQFGKQE PGSNEEIKEFAAGYNVKFDM FSKICVNGDDAHPLWKWMKI QPKGKGILGNAIKWNFTKFL IDKNGCVVKRYGPMEEPLVI EKDLPHYF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2). |
Additional Info
| Tissue Specificity | Present primarily in testis. Expressed in platelets (at protein level). |
| Function | Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins. Can also reduce cholesterol hydroperoxide and thymine hydroperoxide. Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation. Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis. The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures. May be required to protect cells from the toxicity of ingested lipid hydroperoxides. Required for normal sperm development and male fertility. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion. Plays a role of glutathione peroxidase in platelets in the arachidonic acid metabolism. Reduces hydroperoxy ester lipids formed by a 15-lipoxygenase that may play a role as down-regulator of the cellular 15-lipoxygenase pathway. Can reduce fatty acid-derived hydroperoxides. Can also reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide. |
| Protein Name | Phospholipid Hydroperoxide Glutathione Peroxidase Gpx4PhgpxGlutathione Peroxidase 4Gpx-4Gshpx-4 |
| Database Links | Reactome: R-HSA-2142688Reactome: R-HSA-2142712Reactome: R-HSA-2142770Reactome: R-HSA-9018676 P36969-2Reactome: R-HSA-9018896 P36969-2Reactome: R-HSA-9020265 P36969-2Reactome: R-HSA-9023661 P36969-2 |
| Cellular Localisation | Isoform Mitochondrial: MitochondrionIsoform Cytoplasmic: Cytoplasm |
| Alternative Antibody Names | Anti-Phospholipid Hydroperoxide Glutathione Peroxidase Gpx4 antibodyAnti-Phgpx antibodyAnti-Glutathione Peroxidase 4 antibodyAnti-Gpx-4 antibodyAnti-Gshpx-4 antibodyAnti-GPX4 antibody |
Information sourced from Uniprot.org