• Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2A subunit.
  • Immunostaining of 21 DIV nucleofected mouse striatal neuron (green) co-cultured with cortical neurons showing nice punctate labeling of the N-terminal NR2A subunit (red, 1:500) in both the medium spiny neurons and the large pyramidal cell in the upper right. Photo courtesy of Dr. A. J. Milnerwood, Dr. Lynn Raymond Lab, University of British Columbia.

Anti-GRIN2A antibody (N-Term) (STJA0003713)

SKU:
STJA0003713-100

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/ICC
Reactivity: Mouse/Rat/Bovine/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NMDAR2A (N-Term) is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC
ICC 1:500
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Grin2a
Gene ID: 24409
Uniprot ID: NMDE1_RAT
Immunogen Region: N-Term
Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2A subunit conjugated to KLH.
Tissue Specificity Detected in brain cortex, olfactory bulb, hippocampus including the dentate gyrus, striatum, thalamus, superior colliculus, inferior colliculus, midbrain and cerebellum (at protein level). Detected in brain cortex, hypothalamus and cerebellum.
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning.
Protein Name Glutamate Receptor Ionotropic - Nmda 2a
Glun2a
Glutamate Nmda Receptor Subunit Epsilon-1
N-Methyl D-Aspartate Receptor Subtype 2a
Nmdar2a
Nr2a
Database Links Reactome: R-RNO-438066
Reactome: -RNO-8849932
Reactome: -RNO-9609736
Cellular Localisation Cell Projection
Dendritic Spine
Cell Membrane
Multi-Pass Membrane Protein
Synapse
Postsynaptic Cell Membrane
Cytoplasmic Vesicle Membrane
Expression At The Dendrite Cell Membrane And At Synapses Is Regulated By Sorcs2 And The Retromer Complex
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 2a antibody
Anti-Glun2a antibody
Anti-Glutamate Nmda Receptor Subunit Epsilon-1 antibody
Anti-N-Methyl D-Aspartate Receptor Subtype 2a antibody
Anti-Nmdar2a antibody
Anti-Nr2a antibody
Anti-Grin2a antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance