• Immunofluorescence analysis of HeLa cells using GRK2 Rabbit monoclonal antibody (STJ11102083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts from wild type (WT) and GRK2 knockout (KO) HeLa cells, using GRK2 antibody (STJ11102083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using GRK2 Rabbit monoclonal antibody (STJ11102083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-GRK2 antibody (590-689) [S3MR] (STJ11102083)

SKU:
STJ11102083

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-GRK2 (590-689) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK2
Gene ID: 156
Uniprot ID: ARBK1_HUMAN
Immunogen Region: 590-689
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 590-689 of human GRK2 (P25098).
Immunogen Sequence: WRGEGEAPQSLLTMEEIQSV EETQIKERKCLLLKIRGGKQ FILQCDSDPELVQWKKELRD AYREAQQLVQRVPKMKNKPR SPVVELSKVPLVQRGSANGL
Tissue Specificity Expressed in peripheral blood leukocytes.
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Inhibits relaxation of airway smooth muscle in response to blue light.
Protein Name Beta-Adrenergic Receptor Kinase 1
Beta-Ark-1
G-Protein Coupled Receptor Kinase 2
Database Links Reactome: R-HSA-111933
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-5635838
Reactome: R-HSA-8856825
Cellular Localisation Cytoplasm
Cell Membrane
Postsynapse
Presynapse
Alternative Antibody Names Anti-Beta-Adrenergic Receptor Kinase 1 antibody
Anti-Beta-Ark-1 antibody
Anti-G-Protein Coupled Receptor Kinase 2 antibody
Anti-GRK2 antibody
Anti-ADRBK1 antibody
Anti-BARK antibody
Anti-BARK1 antibody

Information sourced from Uniprot.org

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