• Immunofluorescence analysis of L929 cells using NMDAR2A Rabbit polyclonal antibody (STJ111046) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of H9C2 cells using NMDAR2A Rabbit polyclonal antibody (STJ111046) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using NMDAR2A Rabbit polyclonal antibody (STJ111046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-GRIN2A antibody (1130-1400) (STJ111046)

SKU:
STJ111046

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Host: Rabbit
Applications: WB/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-GRIN2A (1130-1400) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRIN2A
Gene ID: 2903
Uniprot ID: NMDE1_HUMAN
Immunogen Region: 1130-1400
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1130-1400 of human NMDAR2A (NP_000824.1).
Immunogen Sequence: LDPPQFVENVTLPENVDFPD PYQDPSENFRKGDSTLPMNR NPLHNEEGLSNNDQYKLYSK HFTLKDKGSPHSETSERYRQ NSTHCRSCLSNMPTYSGHFT MRSPFKCDACLRMGNLYDID EDQMLQETGNPATGEQVYQQ DWAQNNALQLQKNKLRISRQ HSYDNIVDKPRELDLSRPSR SISLKDRERLLEGNFYGSLF SVPSSKLSGKKSSLFPQGLE DSKRSKSLLPDHTSDNPFL
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition.channels containing GRIN1 and GRIN2A have lower sensitivity to glutamate and faster deactivation kinetics than channels formed by GRIN1 and GRIN2B. Contributes to the slow phase of excitatory postsynaptic current, long-term synaptic potentiation, and learning.
Protein Name Glutamate Receptor Ionotropic - Nmda 2a
Glun2a
Glutamate Nmda Receptor Subunit Epsilon-1
N-Methyl D-Aspartate Receptor Subtype 2a
Nmdar2a
Nr2a
Hnr2a
Database Links Reactome: R-HSA-438066
Reactome: R-HSA-6794361
Reactome: R-HSA-8849932
Reactome: R-HSA-9022699
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Projection
Dendritic Spine
Cell Membrane
Multi-Pass Membrane Protein
Synapse
Postsynaptic Cell Membrane
Cytoplasmic Vesicle Membrane
Expression At The Dendrite Cell Membrane And At Synapses Is Regulated By Sorcs2 And The Retromer Complex
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 2a antibody
Anti-Glun2a antibody
Anti-Glutamate Nmda Receptor Subunit Epsilon-1 antibody
Anti-N-Methyl D-Aspartate Receptor Subtype 2a antibody
Anti-Nmdar2a antibody
Anti-Nr2a antibody
Anti-Hnr2a antibody
Anti-GRIN2A antibody
Anti-NMDAR2A antibody

Information sourced from Uniprot.org

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