Anti-Green fluorescent protein antibody (STJA0040712)
SPECIFICATIONS
ClonalityPolyclonal
HostChicken
ConjugationUnconjugated
IsotypeIgY
ImmunogenRecombinant AcGFP protein expressed in and purified from E. Coli.
General Information
| Short Description | Chicken polyclonal anti-Green fluorescent protein for use in ICC and WB in Species Independent samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/WB |
| Host | Chicken |
| Reactivity | Species Independent |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgY |
| Conjugation | Unconjugated |
| Purification | Affinity-purified |
| Dilution Range | WB 1:1000-1:5000ICC 1:1000-1:5000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquo |
Target Information
| Immunogen | Recombinant AcGFP protein expressed in and purified from E. Coli. |
| Immunogen Sequence | Jellyfish |
| Specificity | Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells. Specific for GFP, does not cross-react with mCherry. |
Additional Info
| Background | Our Anti-Green fluorescent protein (GFP) chicken polyclonal primary antibody detects species independent Green fluorescent protein (GFP) , and is affinity-purified. It is validated for use in ICC, WB. |
Information sourced from Uniprot.org