Protein Localisation | Research Area | St John's Laboratory

Western blot analysis of lysates from wild type (WT) and GSTK1 knockout (KO) 293T cells, using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11100839) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-GSTK1 antibody (1-226) (STJ11100839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of Hela cells using [KO Validated] GSTK1 Rabbit polyclonal antibody (STJ11104362) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-GSTK1 antibody (1-226) (STJ11104362)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using GSTK1 Rabbit polyclonal antibody (STJ23885) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-GSTK1 antibody (1-226) (STJ23885)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GSTK1 antibody (138-188 aa) (STJ195875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-HACL1 antibody (200-300 aa) (STJ11106391)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-HACL1 antibody (323-373 aa) (STJ196113)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of paraffin-embedded rat liver using HAO1/GOX Rabbit polyclonal antibody (STJ28553) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HAO1 antibody (1-370) (STJ28553)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Polyclonal Antibody. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-HAO1 antibody (STJ197373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£48.00 - £252.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, Chk2 (phospho Thr68) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HAO1 antibody [Mix] (STJ97395)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot analysis of lysates from Mouse kidney, using HAO2 Rabbit polyclonal antibody (STJ117353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HAO2 antibody (1-351) (STJ117353)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of various cell lines, using HMGCL antibody (STJ110355) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 15s.

Anti-HMGCL antibody (1-325) (STJ110355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Anti-HMGCL antibody (C-Term) (STJA0010324)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded Rat spleen using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ113706) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody (1-265) (STJ113706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of Raw264. 7 cells, using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11101600) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-HMOX1 antibody (1-265) [S0MR] (STJ11101600)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

Choose Options

Western blot analysis of various lysates, using HemeOxygenase1 (HO-1/HMOX1) antibody (STJ11104601) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HMOX1 antibody (1-265) [S4601RM] (STJ11104601)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of NIH-3T3 using Heme Oxygenase 1 (HO-1/HMOX1) antibody (STJ11104351) at dilution of 1 : 50 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ11104351)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ113462) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-HMOX1 antibody (1-288) (STJ113462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£112.50 - £524.50

Choose Options

Immunofluorescence analysis of HepG2 cells using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit polyclonal antibody (STJ116419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-HMOX1 antibody (1-288) (STJ116419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of 3T3 Cell lysate using HO-1 mouse Monoclonal monoclonal antibody diluted at 1:2,000.

Anti-HMOX1 antibody [Mix] (STJ196663)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB/IF

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

Choose Options

Immunohistochemistry analysis of Heme Oxygenase 1 (HO-1/HMOX1) in paraffin-embedded mouse liver using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit monoclonal antibody (STJ11105129) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-HMOX1 antibody [S5129RM] (STJ11105129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

Choose Options

Western blot analysis of various lysates using HSCB Rabbit polyclonal antibody (STJ118406) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-HSCB antibody (30-235) (STJ118406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

Choose Options

Anti-HSCB antibody (STJA0008832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

Choose Options

Western blot analysis of HuvEc, K562 cells using 17 Beta-HSD4 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSD17B4 antibody (41-90 aa) (STJ96751)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Anti-HSP60 antibody [HSP60-1] (STJ16102614)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/WB/IF/IHC

Reactivity :

Chicken/Cow/Dog/Hamster/Human/Monkey/Mouse/Pig/Rabbit/Rat/Sheep

£287.50 - £1,324.50

Choose Options

Anti-HSP60 antibody [SM5037] (STJA0005037)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

Choose Options

Immunofluorescence analysis of U2OS cells using Grp75/MOT/Grp75/MOT/HSPA9 antibody (STJ24100). Blue: DAPI for nuclear staining.

Anti-HSPA9 antibody (380-679) (STJ24100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of extracts of various cell lines, using Grp75/MOT/HSPA9 Rabbit monoclonal antibody (STJ11101306) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA9 antibody (580-679) [S6MR] (STJ11101306)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g HSP60/HSPD1 antibody (STJ24105). Western blot was performed from the immunoprecipitate using HSP60/HSPD1 antibody (STJ24105) at a dilution of 1:1000.

Anti-HSPD1 antibody (27-240) (STJ24105)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Confocal imaging of HeLa cells using HSP60/HSPD1 Rabbit monoclonal antibody (STJ11101241, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.

Anti-HSPD1 antibody (350-450) [S1MR] (STJ11101241)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Wheat

£146.50 - £642.50

Choose Options

Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HSP60 antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Predicted band size: 60 kDa

Anti-HSPD1 antibody (500-573 aa) [ABT186] (STJ197164)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

Choose Options

Immunofluorescence analysis of Hela cell. 1, HSP60 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-HSPD1 antibody (511-560 aa) (STJ93621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Anti-HSPD1 antibody (Full Length) [1C7] (STJA0003845)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Zebrafish

£454.50

Choose Options

Anti-HSPD1 antibody (N-Term) [M438] (STJA0005440)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

Choose Options

Western blot detection of HSP60 in pc-12, COS7, 3T3, Hela Rat kidney, Rat Skeletal muscle Rat lung and T47D cell lysates using HSP60 antibody (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-HSPD1 antibody (STJ99294)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

Choose Options

Western blot analysis of 1) 293 Cell, 2) Mouse Kidney Tissue, 3) Rat Heart Tissue lysate using HSP60 mouse monoclonal antibody diluted at 1:2,000.

Anti-HSPD1 antibody [2D3] (STJ196695)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

Choose Options

Western blot analysis using HSP60 monoclonal antibody against T47D (1) , HeLa (2) , HepG2 (3) , A549 (4) , Jurkat (5) , HEK293 (6) , NIH/3T3 (7) , PC-12 (8) and Cos7 (9) cell lysate.

Anti-HSPD1 antibody [3G8] (STJ98142)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

Choose Options

Western blot detection of Hsp60 in Hela, Raji, Jurkat, C6, C2C12 and 3T3 cell lysates using Hsp60 mouse mAb (1:1000 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-HSPD1 antibody [6C8-G1-B10] (STJ99241)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

Choose Options

Western blot analysis of extracts of various cell lines, using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) Rabbit monoclonal antibody (STJ11101287) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDE antibody (1-100) [S7MR] (STJ11101287)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

Choose Options

Immunofluorescence analysis of A549 cells using Insulin-degrading enzyme (Insulin-degrading enzyme (IDE) ) antibody (STJ24120). Blue: DAPI for nuclear staining.

Anti-IDE antibody (1-250) (STJ24120)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

Choose Options

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Insulin Degrading Enzyme/IDE antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-IDE antibody (56-69 aa) [R07-6D3] (STJA0012660)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human

£264.50

Choose Options

Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, IDE monoclonal antibody (3H4) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-IDE antibody [3H4] (STJ96985)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Hamster

£48.00 - £252.00

Choose Options

Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g IDH1 antibody (STJ115211). Western blot was performed from the immunoprecipitate using IDH1 antibody (STJ115211) at a dilution of 1:1000.

Anti-IDH1 antibody (1-414) (STJ115211)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

Choose Options

Immunofluorescence analysis of PC-12 cells using IDH1 Rabbit polyclonal antibody (STJ24121) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-IDH1 antibody (100-414) (STJ24121)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£112.50 - £524.50

Choose Options

Western blot analysis of 293, HeLa cells using IDH1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-IDH1 antibody (N-Term) (STJ96781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

Choose Options

Formalin-fixed, paraffin-embedded human glioma stained with anti-IHD1 antibody using peroxidase-conjugate and DAB chromogen. Note nuclear and cytoplasmic staining of infiltrating glioma cells whereas normal glial cells are negative

Anti-IDH1 antibody [ZR7] (STJ180518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£202.50 - £814.50

Choose Options

Western blot analysis of extracts of various cell lines, using IDH3G antibody (STJ115696) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-IDH3G antibody (40-170) (STJ115696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

Choose Options

Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-IDH3G antibody (STJ193072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

Choose Options

Confocal imaging of RAW264. 7 LPS and RAW264. 7 cells using iNOS Rabbit monoclonal antibody (STJ11103535, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-iNOS antibody (2-200) [S5MR] (STJ11103535)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

Choose Options