• Western blot analysis of extracts of various cell lines, using DAO antibody (STJ11104697) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
  • Western blot analysis of extracts of various cell lines, using DAO antibody (STJ11104697) at1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-DAO antibody (1-100) [S4697RM] (STJ11104697)

SKU:
STJ11104697

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-D-Amino-Acid Oxidase (1-100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4697RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:2000-1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DAO
Gene ID: 1610
Uniprot ID: OXDA_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DAO (NP_001908.3).
Immunogen Sequence: MRVVVIGAGVIGLSTALCIH ERYHSVLQPLDIKVYADRFT PLTTTDVAAGLWQPYLSDPN NPQEADWSQQTFDYLLSHVH SPNAENLGLFLISGYNLFHE
Function Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Protein Name D-Amino-Acid Oxidase
Daao
Damox
Dao
Database Links Reactome: R-HSA-389661
Reactome: R-HSA-9033241
Cellular Localisation Peroxisome
Alternative Antibody Names Anti-D-Amino-Acid Oxidase antibody
Anti-Daao antibody
Anti-Damox antibody
Anti-Dao antibody
Anti-DAO antibody
Anti-DAMOX antibody

Information sourced from Uniprot.org

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