Immunohistochemistry analysis of ECH1 in paraffin-embedded human placenta using ECH1 Rabbit polyclonal antibody (STJ114810) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ECH1 antibody (1-328) (STJ114810)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ114810
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General Information

Short DescriptionRabbit polyclonal ECH1 (1-328) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolECH1
Gene ID1891
Uniprot IDECH1_HUMAN
Immunogen Region1-328
Immunogen SequenceMAAGIVASRRLRDLLTRRLT GSNYPGLSISLRLTGSSAQE EASGVALGEAPDHSYESLRV TSAQKHVLHVQLNRPNKRNA MNKVFWREMVECFNKISRDA DCRAVVISGAGKMFTAGIDL MDMASDILQPKGDDVARISW YLRDIITRYQETFNVIERCP KPVIAAVHGGCIGGGVDLVT ACDIRYCAQDAFFQVKEVDV GLAADVGTLQRLPKVIGNQS LVNELAFTARKMMADEALG
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).

Additional Info

Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
Protein Name Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial
Database Links Reactome: R-HSA-9033241
Reactome: R-HSA-9837999
Cellular Localisation Mitochondrion
Peroxisome
Alternative Antibody Names Anti-Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial antibody
Anti-ECH1 antibody

Information sourced from Uniprot.org

Citations

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