• Immunohistochemistry analysis of ECH1 in paraffin-embedded human placenta using ECH1 Rabbit polyclonal antibody (STJ114810) at dilution of 1:150 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from Mouse heart using ECH1 Rabbit polyclonal antibody (STJ114810) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using ECH1 Rabbit polyclonal antibody (STJ114810) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ECH1 antibody (1-328) (STJ114810)

SKU:
STJ114810

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECH1 (1-328) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECH1
Gene ID: 1891
Uniprot ID: ECH1_HUMAN
Immunogen Region: 1-328
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human ECH1 (NP_001389.2).
Immunogen Sequence: MAAGIVASRRLRDLLTRRLT GSNYPGLSISLRLTGSSAQE EASGVALGEAPDHSYESLRV TSAQKHVLHVQLNRPNKRNA MNKVFWREMVECFNKISRDA DCRAVVISGAGKMFTAGIDL MDMASDILQPKGDDVARISW YLRDIITRYQETFNVIERCP KPVIAAVHGGCIGGGVDLVT ACDIRYCAQDAFFQVKEVDV GLAADVGTLQRLPKVIGNQS LVNELAFTARKMMADEALG
Function Isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA.
Protein Name Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial
Database Links Reactome: R-HSA-9033241
Cellular Localisation Mitochondrion
Peroxisome
Alternative Antibody Names Anti-Delta(3 -5-Delta(2 -4-Dienoyl-Coa Isomerase - Mitochondrial antibody
Anti-ECH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance