• Confocal immunofluorescence analysis of U2OS cells using CS Polyclonal Antibody (STJ28281) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF-7 cells using CS antibody (STJ28281). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using CS antibody (STJ28281) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CS antibody (167-466) (STJ28281)

SKU:
STJ28281

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-CS (167-466) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CS
Gene ID: 1431
Uniprot ID: CISY_HUMAN
Immunogen Region: 167-466
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 167-466 of human CS (NP_004068.2).
Immunogen Sequence: QLSAAVTALNSESNFARAYA QGISRTKYWELIYEDSMDLI AKLPCVAAKIYRNLYREGSG IGAIDSNLDWSHNFTNMLGY TDHQFTELTRLYLTIHSDHE GGNVSAHTSHLVGSALSDPY LSFAAAMNGLAGPLHGLANQ EVLVWLTQLQKEVGKDVSDE KLRDYIWNTLNSGRVVPGYG HAVLRKTDPRYTCQREFALK HLPNDPMFKLVAQLYKIVPN VLLEQGKAKNPWPNVDAHS
Post Translational Modifications Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.
Protein Name Citrate Synthase - Mitochondrial
Citrate
Si-Synthase
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Citrate Synthase - Mitochondrial antibody
Anti-Citrate antibody
Anti-Si-Synthase antibody
Anti-CS antibody

Information sourced from Uniprot.org

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