• Confocal imaging of HeLa cells using Emerin/EMD Rabbit monoclonal antibody (STJ11102013, dilution 1:100) (Red). The cells were counterstained with Alpha-tubulin (ubiquitous) chain Rabbit monoclonal antibody ( dilution 1:100) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.
  • Immunohistochemistry analysis of Emerin/EMD in paraffin-embedded human colon using Emerin/EMD Rabbit monoclonal antibody (STJ11102013) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Emerin/EMD Rabbit monoclonal antibody (STJ11102013) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EMD antibody (150-254) [S3MR] (STJ11102013)

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STJ11102013

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Emerin (150-254) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S3MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EMD
Gene ID: 2010
Uniprot ID: EMD_HUMAN
Immunogen Region: 150-254
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 150-254 of human Emerin/EMD (P50402).
Immunogen Sequence: RERPMYGRDSAYQSITHYRP VSASRSSLDLSYYPTSSSTS FMSSSSSSSSWLTRRAIRPE NRAPGAGLGQDRQVPLWGQL LLFLVFVIVLFFIYHFMQAE EGNPF
Tissue Specificity Skeletal muscle, heart, colon, testis, ovary and pancreas.
Post Translational Modifications Found in four different phosphorylated forms, three of which appear to be associated with the cell cycle.
Function Stabilizes and promotes the formation of a nuclear actin cortical network. Stimulates actin polymerization in vitro by binding and stabilizing the pointed end of growing filaments. Inhibits beta-catenin activity by preventing its accumulation in the nucleus. Acts by influencing the nuclear accumulation of beta-catenin through a CRM1-dependent export pathway. Links centrosomes to the nuclear envelope via a microtubule association. Required for proper localization of non-farnesylated prelamin-A/C. Together with NEMP1, contributes to nuclear envelope stiffness in germ cells. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
Protein Name Emerin
Database Links Reactome: R-HSA-2980766
Reactome: R-HSA-2995383
Reactome: R-HSA-4419969
Reactome: R-HSA-9013149
Reactome: R-HSA-9013404
Reactome: R-HSA-9013405
Reactome: R-HSA-9013408
Reactome: R-HSA-9013423
Reactome: R-HSA-9609523
Cellular Localisation Nucleus Inner Membrane
Single-Pass Membrane Protein
Nucleoplasmic Side
Nucleus Outer Membrane
Colocalized With Banf1 At The Central Region Of The Assembling Nuclear Rim
Near Spindle-Attachment Sites
The Accumulation Of Different Intermediates Of Prelamin-A/C (Non-Farnesylated Or Carboxymethylated Farnesylated Prelamin-A/C) In Fibroblasts Modify Its Localization In The Nucleus
Alternative Antibody Names Anti-Emerin antibody
Anti-EMD antibody
Anti-EDMD antibody
Anti-STA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance