Metabolism Of Proteins | Research Area | St John's Laboratory

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g TriMethyl-Histone H3-K27 mouse monoclonal antibody antibody (STJ118639) , along with a Goat Anti-mouse IgG (H+L). The CUT&Tag results indicate the enrichment pattern of TriMethyl-Histone H3-K27 in representative gene loci (MYOD1) , as shown in figure.

Anti-Tri-Methyl-Histone H3-K27 antibody [S9MM] (STJ118639)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

$197.78 - $867.38

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23972). Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H4-K20 antibody (STJ23972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

$174.83 - $867.38

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H4-Lys20 antibody (STJ197281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Tri-Methyl-Histone H4-Lys59 antibody (STJ197280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Tri-Methyl-Histone H4-Lys79 antibody (STJ197279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Tri-Methyl-TP53-Lys370 antibody (STJA0005917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates, using TRIAP1 rabbit polyclonal antibody (STJ29452) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRIAP1 antibody (1-76) (STJ29452)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-TRIAP1 antibody (STJA0007880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-TRIM13 antibody (40-310) (STJ116298)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts from wild type (WT) and TRIM25 knockout (KO) 293T (KO) cells, using TRIM25 antibody (STJ11102049) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM25 antibody (100-200) [S9MR] (STJ11102049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of various lysates, using TRIM25 rabbit polyclonal antibody (STJ114804) at 1:1500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM25 antibody (190-630) (STJ114804)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-TRIM25 antibody (201-250 aa) (STJ92843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of TRIM27 in paraffin-embedded Mouse kidney using TRIM27 Rabbit polyclonal antibody (STJ118505) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TRIM27 antibody (1-200) (STJ118505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using TRIM27 antibody (STJ11100927) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TRIM27 antibody (214-513) (STJ11100927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of lysates from U2OS cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRIM27 antibody (34-84 aa) (STJ193470)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using KAP1/TRIM28 antibody (STJ11101703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TRIM28 antibody (250-350) [S3MR] (STJ11101703)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug KAP1/TRIM28 Rabbit polyclonal antibody (STJ25965 1:400). Western blot was performed from the immunoprecipitate using KAP1/TRIM28 Rabbit polyclonal antibody (STJ25965) at a dilition of 1:1000.

Anti-TRIM28 antibody (576-835) (STJ25965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis using TIF1 Beta monoclonal antibody against 293T, HepG2 cell lysate.

Anti-TRIM28 antibody (C-Term) (STJ98563)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat/Dog/Rabbit

$194.40 - $469.80

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Western blot detection of KAP1/TIF1 beta in 293T and HepG2 cell lysates using KAP1/TIF1 beta mouse mAb (1:1000 diluted).Observed band size: 110KDa.

Anti-TRIM28 antibody [4E1-D12-F8] (STJ99057)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

$194.40 - $469.80

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Immunofluorescence analysis of U2OS cells using TRIM4 Rabbit polyclonal antibody (STJ118367) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-TRIM4 antibody (1-280) (STJ118367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates using TRMT112 Rabbit polyclonal antibody (STJ116522) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TRMT112 antibody (1-125) (STJ116522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from SW480 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TRMT112 antibody (65-115 aa) (STJ195606)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunohistochemistry analysis of TRRAP in paraffin-embedded human esophageal cancer using TRRAP Rabbit polyclonal antibody (STJ11101534) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TRRAP antibody (3660-3859) (STJ11101534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TRRAP antibody (STJ191546)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF

Reactivity :

Human/Mouse

$64.80 - $340.20

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Immunofluorescence analysis of U-2 OS cells using TSFM Rabbit polyclonal antibody (STJ118534) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TSFM antibody (245-346) (STJ118534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-TSFM antibody (STJA0008641)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry with Anti-TSHB Antibody (STJ503423) at 1:100 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-TSHB antibody (1-50) (STJ503423)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-TSHB antibody (1-50) {Biotin} (STJ503425)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-TSHB antibody (1-50) {FITC} (STJ503426)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of U2OS cells using TSHB antibody (STJ28863). Blue: DAPI for nuclear staining.

Anti-TSHB antibody (21-138) (STJ28863)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Human pituitary adenoma tissue was stained with Anti-TSH (ABT-TSH) Antibody

Anti-TSHB antibody (50-138 aa) [ABT-TSH] (STJ196901)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Western blot analysis of various lysates using TSHB Rabbit polyclonal antibody (STJ116734) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TSHB antibody (C-Term) (STJ116734)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-TSHB antibody (STJ192321)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-TSHB antibody (STJ503424)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$577.13

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Anti-TSHB antibody [5401] (STJ400191)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$602.78 - $775.58

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Anti-TSHB antibody [5404] (STJ400193)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$602.78 - $775.58

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Anti-TSHB antibody [5407] (STJ400195)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$602.78 - $775.58

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Anti-TSHB antibody [5408] (STJ400196)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$602.78 - $775.58

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Anti-TSHB antibody [5409] (STJ400197)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$602.78 - $775.58

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Anti-TSHB antibody {Biotin} (STJ503427)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-TSHB antibody {FITC} (STJ503428)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-TSPAN14 antibody (STJA0008787)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from mouse spleen, using TSPAN15 Rabbit polyclonal antibody (STJ116607) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-TSPAN15 antibody (110-220) (STJ116607)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-TSPAN15 antibody (STJA0009050)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using TSPAN33 Rabbit polyclonal antibody (STJ27195) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TSPAN33 antibody (C-Term) (STJ27195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-TSPAN33 antibody (STJA0008001)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-TSPAN5 antibody (113-163 aa) (STJ197725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using TSTA3 Rabbit polyclonal antibody (STJ26557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-TSTA3 antibody (1-321) (STJ26557)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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