| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PLA2G7 (172-441) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PLA2G7 |
| Gene ID: |
7941 |
| Uniprot ID: |
PAFA_HUMAN |
| Immunogen Region: |
172-441 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 172-441 of human PLA2G7 (NP_005075.3). |
| Immunogen Sequence: |
FIVAAVEHRDRSASATYYFK DQSAAEIGDKSWLYLRTLKQ EEETHIRNEQVRQRAKECSQ ALSLILDIDHGKPVKNALDL KFDMEQLKDSIDREKIAVIG HSFGGATVIQTLSEDQRFRC GIALDAWMFPLGDEVYSRIP QPLFFINSEYFQYPANIIKM KKCYSPDKERKMITIRGSVH QNFADFTFATGKIIGHMLKL KGDIDSNVAIDLSNKASLAF LQKHLGLHKDFDQWDCLIE |
| Tissue Specificity | Plasma. Secreted by macrophages (at protein level). |
| Post Translational Modifications | N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles. |
| Function | Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage. |
| Protein Name | Platelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase1-Alkyl-2-Acetylglycerophosphocholine Esterase2-Acetyl-1-Alkylglycerophosphocholine EsteraseGroup-Viia Phospholipase A2Gviia-Pla2Ldl-Associated Phospholipase A2Ldl-Pla(2Paf 2-Acylhydrolase |
| Database Links | Reactome: R-HSA-422085 |
| Cellular Localisation | SecretedExtracellular SpaceAssociates With Both Ldl And Hdl Particles In PlasmaMainly Associates With Pro-Inflammatory Electronegative Ldl Particles |
| Alternative Antibody Names | Anti-Platelet-Activating Factor Acetylhydrolase antibodyAnti-Paf Acetylhydrolase antibodyAnti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibodyAnti-2-Acetyl-1-Alkylglycerophosphocholine Esterase antibodyAnti-Group-Viia Phospholipase A2 antibodyAnti-Gviia-Pla2 antibodyAnti-Ldl-Associated Phospholipase A2 antibodyAnti-Ldl-Pla(2 antibodyAnti-Paf 2-Acylhydrolase antibodyAnti-PLA2G7 antibodyAnti-PAFAH antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
