Anti-PIGK antibody (331-381 aa) (STJ197966)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human GPI8 at the amino acid range 331-381
General Information
| Short Description | Rabbit polyclonal anti-GPI-anchor transamidase (331-381 aa) for use in WB in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIGK |
| Gene ID | 10026 |
| Uniprot ID | GPI8_HUMAN |
| Immunogen | Synthesized peptide derived from the human GPI8 at the amino acid range 331-381 |
| Immunogen Region | 331-381 aa |
| Specificity | This antibody detects endogenous levels of human GPI8 |
Additional Info
| Function | Catalytic subunit of the glycosylphosphatidylinositol-anchor (GPI-anchor) transamidase (GPI-T) complex that catalyzes the formation of the linkage between a proprotein and a GPI-anchor and participates in GPI anchored protein biosynthesis. Recognizes diverse proproteins at a C-terminal signal peptide (CSP) region that lacks consensus sequence and replaces it with a GPI-anchor via a transamidation reaction. Transamidation catalysis reaction follows a two-phase mechanism. In the acyl-enzyme phase, the carbonyl group of the proproteins's omega-site undergoes a nucleophilic attack forming an enzyme-substrate thioester bond. Followed by a general acid catalysis that allows CSP releasing, regenerating the carbonyl, and forming the acyl-enzyme intermediate. In the GPI-anchor attachment phase, the amino group of the GPI-anchor's ethanolamine phosphate, the one on third mannose (EtNP3), mediates a nucleophilic attack on the carbonyl of the acyl-enzyme intermediate, replacing the CSP, allowing GPI-anchor attachment to the omega-residue, therefore forming the product and freeing the enzyme. |
| Protein Name | Gpi-Anchor TransamidaseGpi-Anchor Transamidase Component Pigk - Catalytic SubunitGpi8 HomologHgpi8Phosphatidylinositol-Glycan Biosynthesis Class K ProteinPig-K |
| Database Links | Reactome: R-HSA-162791 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Gpi-Anchor Transamidase antibodyAnti-Gpi-Anchor Transamidase Component Pigk - Catalytic Subunit antibodyAnti-Gpi8 Homolog antibodyAnti-Hgpi8 antibodyAnti-Phosphatidylinositol-Glycan Biosynthesis Class K Protein antibodyAnti-Pig-K antibodyAnti-PIGK antibodyAnti-GPI8 antibody |
Information sourced from Uniprot.org