• Immunofluorescence analysis of L929 cells using PIGM Rabbit polyclonal antibody (STJ119837) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PIGM in paraffin-embedded Mouse kidney using PIGM Rabbit polyclonal antibody (STJ119837) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PIGM in paraffin-embedded Rat kidney using PIGM Rabbit polyclonal antibody (STJ119837) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PIGM Rabbit polyclonal antibody (STJ119837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.

Anti-PIGM antibody (35-96) (STJ119837)

SKU:
STJ119837

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PIGM (35-96) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PIGM
Gene ID: 93183
Uniprot ID: PIGM_HUMAN
Immunogen Region: 35-96
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 35-96 of human PIGM (NP_660150.1).
Immunogen Sequence: YGVFQDRTLHVRYTDIDYQV FTDAARFVTEGRSPYLRATY RYTPLLGWLLTPNIYLSELF GK
Function Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly.
Protein Name Gpi Mannosyltransferase 1
Gpi Mannosyltransferase I
Gpi-Mt-I
Phosphatidylinositol-Glycan Biosynthesis Class M Protein
Pig-M
Database Links Reactome: R-HSA-162710
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Gpi Mannosyltransferase 1 antibody
Anti-Gpi Mannosyltransferase I antibody
Anti-Gpi-Mt-I antibody
Anti-Phosphatidylinositol-Glycan Biosynthesis Class M Protein antibody
Anti-Pig-M antibody
Anti-PIGM antibody

Information sourced from Uniprot.org

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