• Western blot analysis of lysates from K562 cells treated with etoposide 25uM 24h, using Werner Syndrome Helicase (Phospho-Ser1141) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-WRN-Ser1141 antibody (1107-1156 aa) (STJ90561)

SKU:
STJ90561

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Bifunctional 3 5 exonuclease/ATP-dependent helicase WRN-Ser1141 (1107-1156 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: WRN
Gene ID: 7486
Uniprot ID: WRN_HUMAN
Immunogen Region: 1107-1156 aa
Specificity: Phospho-WRN (S1141) Polyclonal Antibody detects endogenous levels of WRN protein only when phosphorylated at S1141.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Werner Syndrome Helicase around the phosphorylation site of Ser1141 at the amino acid range 1107-1156
Function Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers.stably associates with foci elements generating binding sites for RP-A. Plays a role in double-strand break repair after gamma-irradiation.
Protein Name Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase Wrn
Dna Helicase - Recq-Like Type 3
Recq Protein-Like 2
Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent Helicase
Database Links Reactome: R-HSA-174414
Reactome: R-HSA-174437
Reactome: R-HSA-3108214
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5693554
Reactome: R-HSA-5693568
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Chromosome
Gamma-Irradiation Leads To Its Translocation From Nucleoli To Nucleoplasm And Pml Regulates The Irradiation-Induced Wrn Relocation
Localizes To Dna Damage Sites
Alternative Antibody Names Anti-Bifunctional 3'-5' Exonuclease/Atp-Dependent Helicase Wrn antibody
Anti-Dna Helicase - Recq-Like Type 3 antibody
Anti-Recq Protein-Like 2 antibody
Anti-Werner Syndrome Protein Includes - 3'-5' Exonuclease - Atp-Dependent Helicase antibody
Anti-WRN antibody
Anti-RECQ3 antibody
Anti-RECQL2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance