| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PIAS1 (422-651) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PIAS1 |
| Gene ID: |
8554 |
| Uniprot ID: |
PIAS1_HUMAN |
| Immunogen Region: |
422-651 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 422-651 of human PIAS1 (NP_057250.1). |
| Immunogen Sequence: |
VQEVSASYNGVDGCLSSTLE HQVASHHQSSNKNKKVEVID LTIDSSSDEEEEEPSAKRTC PSLSPTSPLNNKGILSLPHQ ASPVSRTPSLPAVDTSYINT SLIQDYRHPFHMTPMPYDLQ GLDFFPFLSGDNQHYNTSLL AAAAAAVSDDQDLLHSSRFF PYTSSQMFLDQLSAGGSTSL PTTNGSSSGSNSSLVSSNSL RESHSHTVTNRSSTDTASIF GIIPDIISLD |
| Tissue Specificity | Expressed in numerous tissues with highest level in testis. |
| Post Translational Modifications | Sumoylated. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB. Mediates the nuclear mobility and localization of MSX1 to the nuclear periphery, whereby MSX1 is brought into the proximity of target myoblast differentiation factor genes. Also required for the binding of MSX1 to the core enhancer region in target gene promoter regions, independent of its sumolyation activity. Capable of binding to the core enhancer region TAAT box in the MYOD1 gene promoter. (Microbial infection) Restricts Epstein-Barr virus (EBV) lytic replication by acting as an inhibitor for transcription factors involved in lytic gene expression. The virus can use apoptotic caspases to antagonize PIAS1-mediated restriction and express its lytic genes. |
| Protein Name | E3 Sumo-Protein Ligase Pias1Dead/H Box-Binding Protein 1E3 Sumo-Protein Transferase Pias1Gu-Binding ProteinGbpProtein Inhibitor Of Activated Stat Protein 1Rna Helicase Ii-Binding Protein |
| Database Links | Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4090294Reactome: R-HSA-4551638Reactome: R-HSA-5696395Reactome: R-HSA-877312 |
| Cellular Localisation | NucleusNucleus SpecklePml BodyCytoplasmCytoskeletonInteraction With Csrp2 May Induce A Partial Redistribution Along The CytoskeletonInteraction With Msx1 Is Required For Localization To The Nuclear Periphery |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias1 antibodyAnti-Dead/H Box-Binding Protein 1 antibodyAnti-E3 Sumo-Protein Transferase Pias1 antibodyAnti-Gu-Binding Protein antibodyAnti-Gbp antibodyAnti-Protein Inhibitor Of Activated Stat Protein 1 antibodyAnti-Rna Helicase Ii-Binding Protein antibodyAnti-PIAS1 antibodyAnti-DDXBP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
