Anti-PIAS4 antibody (451-500 aa) (STJ95085)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human PIAS4 at the amino acid range 451-500
General Information
| Short Description | Rabbit polyclonal anti-E3 SUMO-protein ligase PIAS4 (451-500 aa) for use in WB, IHC, IF and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIAS4 |
| Gene ID | 51588 |
| Uniprot ID | PIAS4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PIAS4 at the amino acid range 451-500 |
| Immunogen Region | 451-500 aa |
| Specificity | PIASy Polyclonal Antibody detects endogenous levels of PIASy protein. |
Additional Info
| Post Translational Modifications | Sumoylated. Lys-35 is the main site of sumoylation. Sumoylation is required for TCF4 sumoylation and transcriptional activation. Represses LEF1 transcriptional activity. SUMO1 is the preferred conjugate. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Mediates sumoylation of ALKBH5, AXIN1, CEBPA, KLF8, GATA2, PARK7, HERC2, MYB, TCF4 and RNF168. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Binds to AT-rich DNA sequences, known as matrix or scaffold attachment regions (MARs/SARs). Catalyzes conjugation of SUMO2 to KAT5 in response to DNA damage, facilitating repair of DNA double-strand breaks (DSBs) via homologous recombination (HR). Mediates sumoylation of PARP1 in response to PARP1 trapping to chromatin. Mediates sumoylation of KLF8, repressiing KLF8 transcriptional activity and cell cycle progression into G(1) phase. Sumoylates ALKBH5 downstream of MAPK8/JNK1 and MAPK9/JNK2 in response to reactive oxygen species (ROS), inhibiting ALKBH5 RNA demethylase activity. |
| Protein Name | E3 Sumo-Protein Ligase Pias4PiasyProtein Inhibitor Of Activated Stat Protein 4Protein Inhibitor Of Activated Stat Protein GammaPias-Gamma |
| Database Links | Reactome: R-HSA-196791Reactome: R-HSA-3108214Reactome: R-HSA-3232118Reactome: R-HSA-3232142Reactome: R-HSA-3899300Reactome: R-HSA-4085377Reactome: R-HSA-4090294Reactome: R-HSA-4615885Reactome: R-HSA-4755510Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-69473 |
| Cellular Localisation | NucleusPml BodyColocalizes With Sumo1 And Tcf7l2/Tcf4 And Lef1 In A Subset Of Pml (Promyelocytic Leukemia) Nuclear Bodies |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias4 antibodyAnti-Piasy antibodyAnti-Protein Inhibitor Of Activated Stat Protein 4 antibodyAnti-Protein Inhibitor Of Activated Stat Protein Gamma antibodyAnti-Pias-Gamma antibodyAnti-PIAS4 antibodyAnti-PIASG antibody |
Information sourced from Uniprot.org