Anti-PIAS3 antibody (452-628) (STJ29140)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PIAS3 (452-628) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PIAS3 |
| Gene ID | 10401 |
| Uniprot ID | PIAS3_HUMAN |
| Immunogen Region | 452-628 |
| Immunogen Sequence | IESSSDEEDLPPTKKHCSVT SAAIPALPGSKGVLTSGHQP SSVLRSPAMGTLGGDFLSSL PLHEYPPAFPLGADIQGLDL FSFLQTESQHYGPSVITSLD EQDALGHFFQYRGTPSHFLG PLAPTLGSSHCSATPAPPPG RVSSIVAPGGALREGHGGPL PSGPSLTGCRSDIISLD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 452-628 of human PIAS3 (NP_006090.2). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Post Translational Modifications | Sumoylated. |
| Function | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus. |
| Protein Name | E3 Sumo-Protein Ligase Pias3E3 Sumo-Protein Transferase Pias3Protein Inhibitor Of Activated Stat Protein 3 |
| Database Links | Reactome: R-HSA-3232118Reactome: R-HSA-3899300Reactome: R-HSA-4090294Reactome: R-HSA-4615885Reactome: R-HSA-4755510Reactome: R-HSA-5696395 |
| Cellular Localisation | CytoplasmNucleusNucleus SpeckleColocalizes With Mitf In The NucleusColocalizes With Gfi1 In Nuclear DotsColocalizes With Sumo1 In Nuclear Granules |
| Alternative Antibody Names | Anti-E3 Sumo-Protein Ligase Pias3 antibodyAnti-E3 Sumo-Protein Transferase Pias3 antibodyAnti-Protein Inhibitor Of Activated Stat Protein 3 antibodyAnti-PIAS3 antibody |
Information sourced from Uniprot.org