• Western blot analysis of lysates from Human serum, using PLA2G7 Rabbit polyclonal antibody (STJ115803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PLA2G7 antibody (51-240) (STJ115803)

SKU:
STJ115803

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PLA2G7 (51-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PLA2G7
Gene ID: 7941
Uniprot ID: PAFA_HUMAN
Immunogen Region: 51-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 51-240 of human PLA2G7 (NP_005075.3).
Immunogen Sequence: FGQTKIPRGNGPYSVGCTDL MFDHTNKGTFLRLYYPSQDN DRLDTLWIPNKEYFWGLSKF LGTHWLMGNILRLLFGSMTT PANWNSPLRPGEKYPLVVFS HGLGAFRTLYSAIGIDLASH GFIVAAVEHRDRSASATYYF KDQSAAEIGDKSWLYLRTLK QEEETHIRNEQVRQRAKECS QALSLILDID
Tissue Specificity Plasma. Secreted by macrophages (at protein level).
Post Translational Modifications N-glycosylated. Macrophage-derived PLA2G7 carries sialylated complex-type N-glycans that hinder its binding to HDL particles.
Function Lipoprotein-associated calcium-independent phospholipase A2 involved in phospholipid catabolism during inflammatory and oxidative stress response. At the lipid-aqueous interface, hydrolyzes the ester bond of fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity). Specifically targets phospholipids with a short-chain fatty acyl group at sn-2 position. Can hydrolyze phospholipids with long fatty acyl chains, only if they carry oxidized functional groups. Hydrolyzes and inactivates platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), a potent pro-inflammatory signaling lipid that acts through PTAFR on various innate immune cells. Hydrolyzes oxidatively truncated phospholipids carrying an aldehyde group at omega position, preventing their accumulation in low-density lipoprotein (LDL) particles and uncontrolled pro-inflammatory effects. As part of high-density lipoprotein (HDL) particles, can hydrolyze phospholipids having long-chain fatty acyl hydroperoxides at sn-2 position and protect against potential accumulation of these oxylipins in the vascular wall. Catalyzes the release from membrane phospholipids of F2-isoprostanes, lipid biomarkers of cellular oxidative damage.
Protein Name Platelet-Activating Factor Acetylhydrolase
Paf Acetylhydrolase
1-Alkyl-2-Acetylglycerophosphocholine Esterase
2-Acetyl-1-Alkylglycerophosphocholine Esterase
Group-Viia Phospholipase A2
Gviia-Pla2
Ldl-Associated Phospholipase A2
Ldl-Pla(2
Paf 2-Acylhydrolase
Database Links Reactome: R-HSA-422085
Cellular Localisation Secreted
Extracellular Space
Associates With Both Ldl And Hdl Particles In Plasma
Mainly Associates With Pro-Inflammatory Electronegative Ldl Particles
Alternative Antibody Names Anti-Platelet-Activating Factor Acetylhydrolase antibody
Anti-Paf Acetylhydrolase antibody
Anti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibody
Anti-2-Acetyl-1-Alkylglycerophosphocholine Esterase antibody
Anti-Group-Viia Phospholipase A2 antibody
Anti-Gviia-Pla2 antibody
Anti-Ldl-Associated Phospholipase A2 antibody
Anti-Ldl-Pla(2 antibody
Anti-Paf 2-Acylhydrolase antibody
Anti-PLA2G7 antibody
Anti-PAFAH antibody

Information sourced from Uniprot.org

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