Extracellular Matrix Organisation | Research Area | St John's Laboratory

Western blot analysis of SDC2 Antibody. The lane on the right is blocked with the SDC2 peptide.

Anti-SDC2 antibody (81-130 aa) (STJ95855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Syndecan 3 antibody (STJ11104118) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SDC3 antibody (1-100) [S4118RM] (STJ11104118)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of lysates from HpeG2 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SDC3 antibody (131-181 aa) (STJ195155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-SDC3 antibody (290-380 aa) (STJ11106469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of HepG2 cells using Syndecan-4 Polyclonal Antibody

Anti-SDC4 antibody (145-194 aa) (STJ95856)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of MCF7 cells, using SDC4 antibody (STJ25459) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SDC4 antibody (19-145) (STJ25459)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of various lysates using PAI-1/Serpin E1 Rabbit polyclonal antibody (STJ113996) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 40s.

Anti-SERPINE1 antibody (24-158) (STJ113996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-SERPINE1 antibody (24-158) (STJ116958)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of Rat placenta, using PAI-1/Serpin E1 antibody (STJ27967) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SERPINE1 antibody (24-199) (STJ27967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using PAI-1 Polyclonal Antibody

Anti-SERPINE1 antibody (266-315 aa) (STJ94934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of HepG2 cells, using PAI-1/Serpin E1 antibody (STJ11101633) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SERPINE1 antibody (50-150) [S3MR] (STJ11101633)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Immunofluorescence analysis of NIH/3T3 cells using HSP47/SERPINH1 Rabbit polyclonal antibody (STJ11103839) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SERPINH1 antibody (139-418) (STJ11103839)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SERPINH1 antibody (190-270 aa) (STJ190383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Confocal imaging of NIH/3T3 cells using HSP47/SERPINH1 Rabbit monoclonal antibody (STJ11101393, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SERPINH1 antibody (319-418) [S3MR] (STJ11101393)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of extracts of HeLa cells, using SH3PXD2A antibody (STJ11102477). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST.

Anti-SH3PXD2A antibody (806-1105) (STJ11102477)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-SH3PXD2A antibody (N-Term) (STJ93078)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-SPARC antibody (141-190 aa) (STJ96886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using SPARC Rabbit polyclonal antibody (STJ116705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPARC antibody (18-303) (STJ116705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using SPARC antibody (STJ25671) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SPARC antibody (18-303) (STJ25671)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using SPOCK3 Rabbit polyclonal antibody (STJ114715) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPOCK3 antibody (180-380) (STJ114715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Rat kidney, using Osteopontin Rabbit polyclonal antibody (STJ25680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SPP1 antibody (106-205) (STJ25680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using Osteopontin antibody (STJ25679) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-SPP1 antibody (17-314) (STJ25679)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Osteopontin antibody (STJ11101629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SPP1 antibody (200-300) [S9MR] (STJ11101629)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of various cells using OPN Polyclonal Antibody diluted at 1：500

Anti-SPP1 antibody (261-310 aa) (STJ94832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using Osteopontin antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-SPP1 antibody (287-300 aa) [R08-1C5] (STJA0012170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Hamster/Rat

£264.50

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Anti-SPP1 antibody (N-Term) [M574] (STJA0005500)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Western blot analysis of various lysates using Osteopontin Mouse monoclonal antibody (STJ11104172) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPP1 antibody [S4172MM] (STJ11104172)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-Tenascin-C antibody [DB7] (STJ16100798)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/IHC-F/WB

Reactivity :

Human

£567.50

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Anti-Tenascin-C antibody [EB2] (STJ16100797)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB/ELISA

Reactivity :

Human/Rabbit/Guinea Pig

£567.50

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Anti-TGF beta 1 antibody [SM5017] (STJA0005017)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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Anti-TGF beta 1 antibody [SRM] (STJ11107037)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunofluorescence analysis of U2OS cells using TGF beta 1 Rabbit polyclonal antibody (STJ29875) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TGFB1 antibody (291-390) (STJ29875)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using TGF beta 1 Rabbit polyclonal antibody (STJ117297) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TGFB1 antibody (30-390) (STJ117297)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates of K562 were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TGF β1 (PT2173) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 44kDaObserved band size: 44kDa

Anti-TGFB1 antibody (300-390) [PT2173] (STJA0006239)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human Endometrium. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-TGFB1 antibody (336-385 aa) (STJ95997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-TGFB1 antibody [1F12] (STJ196644)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Western blot analysis of K-562, using TGFbeta1 antibody (STJ11104240) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TGFB1 antibody [S4240RM] (STJ11104240)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded mouse heart using TGFB2 antibody (STJ118666) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-TGFB2 antibody (20-160) (STJ118666)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using TGFB2 antibody (STJ25830) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TGFB2 antibody (20-442) (STJ25830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from Mouse uterus using TGFB2 Rabbit polyclonal antibody (STJ11105662) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:90s.

Anti-TGFB2 antibody (21-414) (STJ11105662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Western blot analysis of mouse-kidney 293T mouse-lung lysate, antibody was diluted at 1000. Secondary antibody was diluted at 1:20000

Anti-TGFB2 antibody (361-410 aa) (STJ98769)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using TGFB3 antibody (STJ110758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-TGFB3 antibody (25-300) (STJ110758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various cells using TGF Beta 3 Polyclonal Antibody

Anti-TGFB3 antibody (261-310 aa) (STJ95999)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using THBS1 antibody (STJ119258) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-THBS1 antibody (150-250) (STJ119258)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Mouse

£112.50 - £524.50

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Immunohistochemistry analysis of THBS1 in paraffin-embedded rat spleen using THBS1 Rabbit polyclonal antibody (STJ25844) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-THBS1 antibody (19-352) (STJ25844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Thrombospondin 1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-THBS1 antibody (547-1170 aa) [R06-5F3] (STJA0012618)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£264.50

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-THBS1 antibody (STJ196535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-TIMP-1 antibody [2A5] (STJ16100714)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-P/WB

Reactivity :

Human

£771.50

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