Anti-DMD antibody (3414-3685 aa) (STJ11107224)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal DMD (3414-3685 aa) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DMD |
| Gene ID | 1756 |
| Uniprot ID | DMD_HUMAN |
| Immunogen Region | 3414-3685 aa |
| Immunogen Sequence | NFWPVDSAPASSPQLSHDDT HSRIEHYASRLAEMENSNGS YLNDSISPNESIDDEHLLIQ HYCQSLNQDSPLSQPRSPAQ ILISLESEERGELERILADL EEENRNLQAEYDRLKQQHEH KGLSPLPSPPEMMPTSPQSP RDAELIAEAKLLRQHKGRLE ARMQILEDHNKQLESQLHRL RQLLEQPQAEAKVNGTTVSS PSTSLQRSDSSQPMLLRVVG SQTSDSMGEEDLLSPPQDT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 3414-3685 of human DMD (NP_003997.2). |
Additional Info
| Tissue Specificity | Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells. |
| Function | Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission. |
| Protein Name | Dystrophin |
| Database Links | Reactome: R-HSA-3000171Reactome: R-HSA-390522Reactome: R-HSA-9913351 |
| Cellular Localisation | Cell MembraneSarcolemmaPeripheral Membrane ProteinCytoplasmic SideCytoplasmCytoskeletonPostsynaptic Cell MembraneIn Muscle CellsSarcolemma Localization Requires The Presence Of Ank2While Localization To Costameres Requires The Presence Of Ank3Localizes To Neuromuscular Junctions (Nmjs)In Adult MuscleNmj Localization Depends Upon Ank2 PresenceBut Not In Newborn Animals |
| Alternative Antibody Names | Anti-Dystrophin antibodyAnti-DMD antibody |
Information sourced from Uniprot.org