• Western blot analysis of extracts of various cell lines, using Dystrophin antibody (STJ23394) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded Human skeletal muscle using Dystrophin rabbit polyclonal antibody (STJ23394) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse heart using Dystrophin rabbit polyclonal antibody (STJ23394) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat heart using Dystrophin rabbit polyclonal antibody (STJ23394) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of Human skeletal muscle cells using Dystrophin rabbit polyclonal antibody (STJ23394) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse skeletal muscle cells using Dystrophin rabbit polyclonal antibody (STJ23394) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-DMD antibody (346-635) (STJ23394)

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STJ23394

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DMD (346-635) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DMD
Gene ID: 1756
Uniprot ID: DMD_HUMAN
Immunogen Region: 346-635
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 346-635 of human Dystrophin (NP_003997.2).
Immunogen Sequence: TALEEVLSWLLSAEDTLQAQ GEISNDVEVVKDQFHTHEGY MMDLTAHQGRVGNILQLGSK LIGTGKLSEDEETEVQEQMN LLNSRWECLRVASMEKQSNL HRVLMDLQNQKLKELNDWLT KTEERTRKMEEEPLGPDLED LKRQVQQHKVLQEDLEQEQV RVNSLTHMVVVVDESSGDHA TAALEEQLKVLGDRWANICR WTEDRWVLLQDILLKWQRLT EEQCLFSAWLSEKEDAVNK
Tissue Specificity Expressed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma. Expressed in brain, muscle, kidney, lung and testis. Most tissues contain transcripts of multiple isoforms. Isoform 15: Only isoform to be detected in heart and liver and is also expressed in brain, testis and hepatoma cells.
Function Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
Protein Name Dystrophin
Database Links Reactome: R-HSA-3000171
Reactome: R-HSA-390522
Cellular Localisation Cell Membrane
Sarcolemma
Peripheral Membrane Protein
Cytoplasmic Side
Cytoplasm
Cytoskeleton
Postsynaptic Cell Membrane
In Muscle Cells
Sarcolemma Localization Requires The Presence Of Ank2
While Localization To Costameres Requires The Presence Of Ank3
Localizes To Neuromuscular Junctions (Nmjs)
In Adult Muscle
Nmj Localization Depends Upon Ank2 Presence
But Not In Newborn Animals
Alternative Antibody Names Anti-Dystrophin antibody
Anti-DMD antibody

Information sourced from Uniprot.org

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