Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from MCF7 cells, using Phospho-TSC2-T1462 Rabbit pAb (STJ11101006) at 1:1000 dilution. MCF7 cells were treated by IGF-1 (50 ng/ml) at 37 °C for 5 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-TSC2-T1462 antibody (STJ11101006)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of 293 using p-Tuberin (T1462) antibody.

Anti-Phospho-TSC2-Thr1462 antibody (1428-1477 aa) (STJ91128)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of TSC2 (Phospho-Tyr1571) Antibody. The lane on the right is blocked with the TSC2 (Phospho-Tyr1571) peptide.

Anti-Phospho-TSC2-Tyr1571 antibody (1537-1586 aa) (STJ91276)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-TTK-Thr676 antibody (642-691 aa) (STJ91222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£367.50

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Western blot analysis of lysates from Rat brain, using TUBA1/3/4 (Phospho-Tyr272) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-Tubulin Alpha 1/3/4-Tyr272 antibody (238-287 aa) (STJ90662)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-TYK2-Tyr1054/1055 antibody (STJ196462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-TYK2-Tyr292 antibody (STJA0009867)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-TYK2-Tyr292 antibody (STJA0009868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Tyk2-Y1054/1055 Rabbit pAb (STJ113493) at 1:1000 dilution. NIH/3T3 cells were treated by IFN-Alpha (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Tyk2-Y1054/1055 antibody (STJ113493)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £642.50

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Anti-Phospho-Tyk2-Y292 antibody (STJ11103717)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-Tyrosine Hydroxylase-S19 antibody (STJ11107634)

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from 293 cells, using Phospho-Tyrosine Hydroxylase-S31 Rabbit pAb (STJ11107621). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-Tyrosine Hydroxylase-S31 antibody (STJ11107621)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from PC-3 cells using Phospho-Ubiquitin-Ser65 Rabbit polyclonal antibody (STJ11105848) at 1:700 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone (30 uM) at 37 °C for 6 hours after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Enhanced Kit. Exposuretime: 90s.

Anti-Phospho-Ubiquitin-Ser65 antibody (STJ11105848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using UBF (Phospho-Ser484) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-UBTF-Ser484 antibody (451-500 aa) (STJ90997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-UGDH-Y473 antibody (STJ11107718)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various lysates using Phospho-ULK1-S467 Rabbit pAb (STJ11103776) at 1:1000 dilution. HeLa and Jurkat cells were treated by Serum-starvation overnight at 37 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-ULK1-S467 antibody (STJ11103776)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-ULK1-S555 Rabbit pAb (STJ116381) at 1:1000 dilution. 293T cells were treated by Oligomycin (0. 5 uM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ULk1-S555 antibody (STJ116381)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-ULK1-S638 Rabbit pAb (STJ11103735) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ULK1-S638 antibody (STJ11103735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of lysates from A-431 cells, using Phospho-ULK1-S757 Rabbit pAb (STJ116374) at 1:500 dilution. A-431 cells were treated by EGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ULk1-S757 antibody (STJ116374)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Mesenchymal stromal cells ameliorate diabetes-induced muscle atrophy through exosomes by enhancing AMPK/ULK1-mediated autophagy Journal of Cachexia Sarcopenia and Muscle Li Chen WB mouse tibialis anterior (TA) muscle

Anti-Phospho-ULK1-Ser555 antibody (STJ196463)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of various cell lysis (provided by our customer). primary Antibody was diluted at 1:1000. Secondary antibody (catalog#:STJS000791 was diluted at 1:10000

Anti-Phospho-ULK1-Ser638 antibody (STJ196464)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Anti-Phospho-ULK1-Ser757 antibody (STJ196465)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1 HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-USF1-Thr153 antibody (STJA0009960)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of MCF7 cell. 1, primary Antibody was diluted at 1:100 (4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - AFluor 594 Secondary antibody (catalog No: STJS000476) was diluted at 1:500 (room temperature, 50min).

Anti-Phospho-USP28-Ser67 antibody (STJA0009968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of various, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-USP28-Ser714 antibody (STJA0009969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-VAMP4-Ser30 antibody (STJA0009993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-VANGL2-S5/S8 antibody [S5796RM] (STJ11105796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-VANGL2-S79/S82/S84 Rabbit mAb (STJ11103744) at1:1000 dilution. HeLa and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VANGL2-S79/S82/S84 antibody [S4MR] (STJ11103744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Anti-Phospho-VANGL2-T78/S79 antibody [S2MR] (STJ11103742)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb (STJ11103743) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-VANGL2-T78/S79/S82 antibody [S3MR] (STJ11103743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (STJ116383) at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 0. 8s.

Anti-Phospho-VASP-S157 antibody (STJ116383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105906) at 1:4000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VASP-S157 antibody [S5906RM] (STJ11105906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105907) at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-VASP-S157 antibody [S5907RM] (STJ11105907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-VASP-S239 Rabbit pAb (STJ22444) at 1:1000 dilution. C6 cells were treated by Forskolin (10 uM) at 37 °C for 1 hour after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VASP-S239 antibody (STJ22444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of various cells using Phospho-VASP (S157) Polyclonal Antibody

Anti-Phospho-VASP-Ser157 antibody (124-173 aa) (STJ90431)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human tonsil. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VASP-Ser157 antibody (STJ90777)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from RAW264.7 cells, using VASP (Phospho-Ser238) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VASP-Ser238 antibody (206-255 aa) (STJ90432)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-VASP-Ser239 antibody (STJ196466)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-VASP-Thr-278 antibody (STJA0005634)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VASP-Thr278 antibody (STJ91280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-VAV1-Tyr160 antibody (131-180 aa) (STJ91129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from RAW264.7 cells, using VAV1 (Phospho-Tyr174) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-VAV1-Tyr174 antibody (141-190 aa) (STJ90434)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-VAV1-Tyr174 antibody (STJ90433)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from Jurkat cells, using Phospho-VAV1-Y174 Rabbit pAb (STJ11105770). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-VAV1-Y174 antibody (STJ11105770)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£129.50 - £642.50

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Anti-Phospho-VAV2-Tyr-142 antibody (STJA0005636)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£367.50

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Anti-Phospho-VAV2-Tyr-172 antibody (STJA0005637)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£367.50

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