Phospho Antibodies | St John's Laboratory

Anti-Phospho-ESR Alpha-S167 antibody (STJ11107642)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts from MCF7 cells, using Phospho-ESR Alpha-S106 antibody (STJ22134). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S106 antibody (STJ22134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

£129.50 - £642.50

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Western blot analysis of extracts of MCF7 cells, using Phospho-ESR Alpha-S118 polyclonal antibody (STJ22133) at 1:1000 dilution. MCF7 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-ESR1-S118 antibody (STJ22133)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of NIH/3T3 cells, using Phospho-ESR Alpha-S118 rabbit monoclonal antibody (STJ11102593) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-ESR1-S118 antibody [S3MR] (STJ11102593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of extracts from MCF7 cells using Phospho-ESR Alpha-S167 antibody (STJ22135). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-ESR1-S167 antibody (STJ22135)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser102) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser102 antibody (71-120 aa) (STJ91254)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-ER Alpha (S104) Polyclonal Antibody

Anti-Phospho-ESR1-Ser104 antibody (71-120 aa) (STJ90262)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of RAT-BRAIN cells using Phospho-ER Alpha (S106) Polyclonal Antibody diluted at 1：500

Anti-Phospho-ESR1-Ser106 antibody (71-120 aa) (STJ90263)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-ER Alpha (S118) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-ESR1-Ser118 antibody (91-140 aa) (STJ90264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Estrogen Receptor-alpha (Phospho-Ser167) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser167 antibody (136-185 aa) (STJ90265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells treated with EGF 200nM 5', using Estrogen Receptor-alpha (Phospho-Ser305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ESR1-Ser305 antibody (276-325 aa) (STJ91336)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western Blot analysis of 1 HeLa cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ESR1-Thr311 antibody (STJA0010024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Phospho-ESR1-Tyr-537 antibody (STJA0005415)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Anti-Phospho-ESR1-Tyr-537 antibody [M545] (STJA0005416)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

£365.50

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Western blot analysis of 293 cells using Phospho-ER Alpha (Y537) Polyclonal Antibody

Anti-Phospho-ESR1-Tyr537 antibody (501-550 aa) (STJ90808)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HepG2 cells using Phospho-ER Beta (S105) Polyclonal Antibody

Anti-Phospho-ESR2-Ser105 antibody (71-120 aa) (STJ90713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ESR2-Ser87 antibody (53-102 aa) (STJ91236)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded human brain tumor. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-ETS1-Ser251 antibody (STJ91277)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various cells using Phospho-Ets-1 (T38) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-ETS1-Thr38 antibody (11-60 aa) (STJ91038)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of C6 cells, using Phospho-EZH2/KMT6-T311 antibody (STJ11105805) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-EZH2-T311 antibody (STJ11105805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£129.50 - £642.50

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Anti-Phospho-EZH2-Thr311 antibody (STJ196302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts from MDA cells, using Phospho-Ezrin-T567 antibody (STJ22136). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-EZR-T567 antibody (STJ22136)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of HELA COLO using Phospho-Ezrin (T566) antibody. Antibody was diluted at 1:1000

Anti-Phospho-EZR-Thr566 antibody (534-583 aa) (STJ90266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-Ezrin (Y146) Polyclonal Antibody

Anti-Phospho-EZR-Tyr146 antibody (111-160 aa) (STJ90569)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from A431 cells treated with EGF 200ng/ml 30', using Ezrin (Phospho-Tyr353) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-EZR-Tyr353 antibody (321-370 aa) (STJ90267)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Ezrin (Phospho-Tyr478) Antibody. The lane on the right is blocked with the Ezrin (Phospho-Tyr478) peptide.

Anti-Phospho-EZR-Tyr478 antibody (446-495 aa) (STJ90570)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of 293T cells, using Phospho-Ezrin-Y353 polyclonal antibody (STJ11101152) at 1:1000 dilution or Ezrin antibody (A0703). 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-EZR-Y353 antibody (STJ11101152)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of PC-12 cells, using Phospho-EZR-T567/RDX-T564/MSN-T558 rabbit polyclonal antibody (STJ11101155) at 1:1000 dilution. PC-12 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-EZR/RDX/MSN-T567/T564/T558 antibody (STJ11101155)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 antibody (STJ11103784) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-Ezrin & Radixin & Moesin-T567 & T564 & T558 antibody (STJ11103784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from RAW264.7 cells treated with TNF 20ng/ml 30', using Coagulation Factor III (Phospho-Ser290) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-F3-Ser290 antibody (246-295 aa) (STJ90893)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of HeLa cells, using Phospho-FADD-S194 polyclonal antibody (STJ11101092) at 1:1000 dilution or FADD antibody (A5819). Hela cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-FADD-S194 antibody (STJ11101092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of JK cells using Phospho-FADD (S191) Polyclonal Antibody

Anti-Phospho-FADD-Ser191 antibody (157-206 aa) (STJ90998)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Mouse

£48.00 - £252.00

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Western blot analysis of MOUSE-KIDNEYmouse brainmouse spleen MOUSE-LUNG using p-FADD (S194) antibody. Antibody was diluted at 1:1000

Anti-Phospho-FADD-Ser194 antibody (159-208 aa) (STJ90788)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western blot of 3T3 cell lysate showing specific immunolabeling of the ~83 kDa FAM129B protein phosphorylated at Ser679/683 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-Phospho-FAM129B-Ser679/683 antibody (STJA0003632)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Non-Human Primates

£454.50

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Western blot analysis of RAT-HEART cells using Phospho-FANCA (S1149) Polyclonal Antibody diluted at 1：500

Anti-Phospho-FANCA-Ser1149 antibody (1121-1170 aa) (STJ91103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FANCD2-Ser222 antibody (188-237 aa) (STJ90790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of mouse spleen, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FANCD2-Thr691 antibody (STJA0010051)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of U2OS cells using Phospho-FANCG-S383 antibody (STJ11100974) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Phospho-FANCG-S383 antibody (STJ11100974)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of K562 cells using Phospho-FANCG (S383) Polyclonal Antibody

Anti-Phospho-FANCG-Ser383 antibody (STJ90615)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser556 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase ( Lambda-Ptase, 800 units/1mg protein for 30 min).

Anti-Phospho-FANCI-Ser556 antibody (STJA0003633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Bovine/Goat/Non-Human Primates/Sheep

£454.50

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Western blot of Hela cell lysate treated with UV (~254nm) for 20 NA showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 min).

Anti-Phospho-FANCI-Ser559 antibody (STJA0003831)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Non-Human Primates

£454.50

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Immunofluorescence analysis of COS7 cells, using FAS (Phospho-Tyr291) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FAS-Tyr291 antibody (257-306 aa) (STJ91337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of 293 and HeLa cells, using Phospho-Fas-Y291 antibody (STJ22139) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. HeLa cells were treated by Doxorubicin (0. 5uM) for 24 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.

Anti-Phospho-FAS-Y291 antibody (STJ22139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-Phospho-FBXW7-T205 antibody (STJ11107696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of lysates from K562 cells treated with PMA 125ng/ml 30', using CD32 (Phospho-Tyr292) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-FCGR2B-Tyr292 antibody (258-307 aa) (STJ90485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western Blot analysis of 1HeLa cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-FEN1-Ser187 antibody (STJA0009947)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunohistochemistry analysis of paraffin-embedded human brain, using FER (Phospho-Tyr402) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FER-Tyr402 antibody (371-420 aa) (STJ90943)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Western Blot analysis of A431 mouse heart using primary antibody at 1:1000 dilution 4°C, overnight. Secondary antibody (STJS000791) was diluted at 1:10000 25°C, 1. 5hours

Anti-Phospho-FER-Tyr714 antibody (STJA0010076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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