• Western blot analysis of extracts of HeLa cells, using Phospho-eIF2 Alpha-S51 polyclonal antibody (STJ116376) at 1:2000 dilution or EIF2S1 antibody (A0764). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells, using 3 Mu g Phospho-eIF2 Alpha-S51 polyclonal antibody (STJ116376). Western blot was performed from the immunoprecipitate using Phospho-eIF2 Alpha-S51 polyclonal antibody (STJ116376) at a dilution of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-eIF2 Alpha-S51 antibody (STJ116376) at dilution of 1:150 (40x lens).
  • Immunofluorescence analysis of HeLa and HeLa treated with CA cells using Phospho-eIF2 Alpha-S51 Rabbit polyclonal antibody (STJ116376) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-eIF2 Alpha-S51 antibody (STJ116376) at dilution of 1:150 (40x lens).
  • Western blot analysis of lysates from HeLa cells, using Phospho-eIF2 Alpha-S51 Rabbit polyclonal antibody (A0764). HeLa cells were treated by Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded rat spleen using Phospho-eIF2 Alpha-S51 antibody (STJ116376) at dilution of 1:150 (40x lens).
  • Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug Phospho-eIF2 Alpha-S51 polyclonal antibody (STJ116376). Western blot was performed from the immunoprecipitate using Phospho-eIF2 Alpha-S51 polyclonal antibody (STJ116376) at a dilition of 1:1000. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight.

Anti-Phospho-EIF2S1-S51 antibody (STJ116376)

SKU:
STJ116376

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Host: Rabbit
Applications: WB/IP/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-eIF2 Alpha-S51 is suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IP 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: EIF2S1
Gene ID: 1965
Uniprot ID: IF2A_HUMAN
Immunogen: A synthetic phosphorylated peptide around S51 of human eIF2 Alpha (NP_004085.1).
Immunogen Sequence: MILLSELSRRRIRSIN
Post Translational Modifications Phosphorylation at Ser-49 and Ser-52 stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF2 between successive rounds of initiation and leading to global inhibition of translation, while concomitantly initiating the preferential translation of integrated stress response (ISR)-specific mRNAs. Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylated.phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation. (Microbial infection) Phosphorylation by vaccinia virus protein E3 and rotavirus A stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF2 between successive rounds of initiation and leading to global inhibition of translation.
Function Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (43S PIC). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex. In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF2-alpha in a global protein synthesis inhibitor, leading to an attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming.
Protein Name Eukaryotic Translation Initiation Factor 2 Subunit 1
Eukaryotic Translation Initiation Factor 2 Subunit Alpha
Eif-2-Alpha
Eif-2a
Eif-2alpha
Eif2-Alpha
Database Links Reactome: R-HSA-156827
Reactome: R-HSA-381042
Reactome: R-HSA-382556
Reactome: R-HSA-72649
Reactome: R-HSA-72695
Reactome: R-HSA-72702
Reactome: R-HSA-72706
Reactome: R-HSA-72731
Reactome: R-HSA-9633012
Reactome: R-HSA-9648895
Cellular Localisation Cytoplasm
Stress Granule
Colocalizes With Nanos3 In The Stress Granules
Alternative Antibody Names Anti-Eukaryotic Translation Initiation Factor 2 Subunit 1 antibody
Anti-Eukaryotic Translation Initiation Factor 2 Subunit Alpha antibody
Anti-Eif-2-Alpha antibody
Anti-Eif-2a antibody
Anti-Eif-2alpha antibody
Anti-Eif2-Alpha antibody
Anti-EIF2S1 antibody
Anti-EIF2A antibody

Information sourced from Uniprot.org

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