• Western blot analysis of extracts of HeLa cells, using Phospho-EIF4EBP1-S65 antibody (STJ22119) at 1:1000 dilution. HeLa cells were treated by IGF-1 (50 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 90s.

Anti-Phospho-EIF4EBP1-S65 antibody (STJ22119)

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STJ22119
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Host: Rabbit
Applications: WB, IP
Reactivity: Human
Short Description: Rabbit polyclonal primary antibody against EIF4EBP1.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification: Affinity purification
Storage Instruction: Store at-20°C. Avoid freeze/thaw cycles.
Isotype: IgG
Dilution Range: WB: 1:500-1:2000
IP: 1:50-1:100
Uniprot ID: 4EBP1_HUMAN
Gene ID: 1978
Gene Symbol: EIF4EBP1
Immunogen: A synthetic phosphorylated peptide around S65 of human EIF4EBP1 (NP_004086.1).
Background This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E) , which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Post Translational Modifications Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E. Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Function Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Database Links Reactome: R-HSA-166208
Protein Name Eukaryotic Translation Initiation Factor 4e-Binding Protein 1
4e-Bp1
Eif4e-Binding Protein 1
Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1
Phas-I
Alternative Names Anti-Eukaryotic Translation Initiation Factor 4e-Binding Protein 1 antibody
Anti-4e-Bp1 antibody
Anti-Eif4e-Binding Protein 1 antibody
Anti-Phosphorylated Heat- And Acid-Stable Protein Regulated By Insulin 1 antibody
Anti-Phas-I antibody
Anti-EIF4EBP1 antibody

Information sourced from uniprot.org

6 months standard