Methyl Antibodies | St John's Laboratory

Dot-blot analysis of all sorts using 5-Methylcytosine (5mC) Rabbit polyclonal antibody (STJ11101496) Line 1：293F cellls transfected with TET2 ProteinLine 2：Another 293F cellls transfected with TET2 ProteinLine 3：293F cellls transfected with GFP-3 ProteinLine 4：DNMT1 knockout (KO) 293F cells

Anti-5-Methyl-Cytosine/5mC antibody (5mC) (STJ11101496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

DB/ELISA

Reactivity :

Species Independent

£110.50 - £505.50

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Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R2 Rabbit polyclonal antibody (STJ11103430) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-Asymmetric Di-Methyl-Histone H3-R2 antibody (STJ11103430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R17 Rabbit pAb (STJ11106419) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Asymmetric DiMethyl-Histone H3-R17 antibody (STJ11106419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Asymmetric DiMethyl-Histone H3-R8 antibody [SRM14495] (STJ11109225)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£118.50 - £521.50

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Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (STJ118453) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-DNMT3A K44me2-K44 antibody (STJ118453)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Di-Methyl-H3-Lys37 antibody (STJA0005912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Di-Methyl-H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16-Lys21 antibody (STJA0005914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-Di-Methyl-Histone H1-Lys25 antibody (STJ197302)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H2B-Lys43 antibody (STJ197300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Di-Methyl-Histone H2B-Lys5 antibody (STJ197299)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U-2 OS cells using DiMethyl-Histone H3-K14 polyclonal antibody (STJ27231) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-K14 antibody (STJ27231)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody (STJ23988) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-K27 antibody (16-37) (STJ23988)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of HeLa, using DiMethyl-Histone H3-K36 antibody (STJ23991) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-K36 antibody (Around K36) (STJ23991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody (STJ23982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Di-Methyl-Histone H3-K4 antibody (2-19) (STJ23982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody (STJ11103475) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-K79 antibody (STJ11103475)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from K-562 cells and DiMethyl-Histone H3-K79 rabbit polyclonal antibody (STJ23994). The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K79 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Di-Methyl-Histone H3-K79 antibody (STJ23994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Di-Methyl-Histone H3-K80 antibody (STJ98849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of HeLa, using DiMethyl-Histone H3-K9 antibody (STJ23985) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Di-Methyl-Histone H3-K9 antibody (2-19) (STJ23985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of NIH-3T3 cells using Di-Methyl-Histone H3 (K10) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-Di-Methyl-Histone H3-Lys10 antibody (STJ97223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Rat Testis Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys14 antibody (STJ197296)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£49.00 - £261.00

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Western blot analysis of Hela, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys18 antibody (STJ197295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£49.00 - £261.00

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Western blot analysis of Hela, diluted at 1) 1:1000 , 2) 1:3000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys27 antibody [3B12] (STJ97003)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Liver Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys36 antibody (STJ197294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

Choose Options

Western blot analysis of 1) Hela, 2) Raw264.7, 3) Rat Brain Tissue, diluted at 1:3, 000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys36 antibody [1E6] (STJ97020)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of MCF-7 cells using Di-Methyl-Histone H3 (K5) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Di-Methyl-Histone H3-Lys5 antibody (STJ90112)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Di-Methyl-Histone H3-Lys79 antibody (STJ197292)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys79 antibody [1A8] (STJ97004)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of Hela, diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys79 antibody [Q7] (STJ96989)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Raw264. 7, 3) Rat Testis Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys9 antibody (STJ197291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) Hela, 2) Rat Heart Tissue, 3) Raw264.7, diluted at 1:2000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Di-Methyl-Histone H3-Lys9 antibody [3C2] (STJ97010)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23965) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R17 antibody (2-22) (STJ23965)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R17 Rabbit polyclonal antibody (STJ23967). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R17 antibody (Around Arg17) (STJ23967)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of U2OS cells using Symmetric DiMethyl-Histone H3-R2 Rabbit polyclonal antibody (STJ23996) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R2 antibody (2-19) (STJ23996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-R2 antibody (Around R2) (STJ24011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R26 Rabbit polyclonal antibody (STJ23963). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R26 antibody (Around Arg26) (STJ23963)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of U-2 OS cells using Symmetric DiMethyl-Histone H3-R26 Rabbit polyclonal antibody (STJ23968) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R26 antibody (Around Arg26) (STJ23968)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 Rabbit polyclonal antibody (STJ23997). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ23997)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

Choose Options

Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R8 antibody (STJ24013) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ24013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Western blot analysis of MOUSE-HEART using Di-Methyl-Histone H3 (K27) Polyclonal Antibody antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Di-Methyl-Histone H3.1-Lys27 antibody (STJ97693)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of 293T cells using DiMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23971). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-K20 antibody (Around K20) (STJ23971)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Anti-Di-Methyl-Histone H4-Lys59 antibody (STJ197283)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Di-Methyl-Histone H4-Lys79 antibody (STJ197282)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23979). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-R3 antibody (2-19) (STJ23979)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H4-R3 Rabbit polyclonal antibody (STJ23973). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H4-R3 antibody (Around Arg3) (STJ23973)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H4-R3 antibody (STJ29402) at 1:1000 dilution.

Anti-Di-Methyl-Histone H4-R3 antibody (STJ29402)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£126.50 - £618.50

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Immunofluorescence analysis of human-breast-cancer tissue. 1, p53 (Di Methyl Lys370) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Di-Methyl-TP53-Lys370 antibody (STJ90115)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from 293T cells, using DiMethyl-eEF1A-K55 Rabbit polyclonal antibody (STJ11103887) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-DiMethyl-eEF1A-K55 antibody (STJ11103887)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£126.50 - £618.50

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Anti-DiMethyl-Histone H3-K36 antibody [SRM13195] (STJ11107925)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£143.50 - £618.50

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