• Western blot analysis of extracts of various cell lines, using Symmetric DiMethyl-Histone H3-R2 antibody (STJ23996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R2 antibody (STJ23996).
  • Immunohistochemistry of paraffin-embedded rat liver using Symmetric DiMethyl-Histone H3-R2 antibody (STJ23996) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human stomach using Symmetric DiMethyl-Histone H3-R2 antibody (STJ23996) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse brain using Symmetric DiMethyl-Histone H3-R2 antibody (STJ23996) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using Symmetric DiMethyl-Histone H3-R2 rabbit polyclonal antibody (STJ23996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Symmetric DiMethyl-Histone H3-R2 rabbit polyclonal antibody (STJ23996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using Symmetric DiMethyl-Histone H3-R2 rabbit polyclonal antibody (STJ23996) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R2 antibody (2-19) (STJ23996)

SKU:
STJ23996

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Symmetric Histone H3-R2 (2-19) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 2-19
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-19 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARTKQTARKSTGGKAPRK
Background This gene belongs to the class II amino-acyl tRNA family. The encoded enzyme catalyzes the transfer of L-serine to tRNA (Ser) and is related to bacterial and yeast counterparts. Multiple alternatively spliced transcript variants have been described but the biological validity of all variants is unknown.

Information sourced from Uniprot.org

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