• Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011).
  • Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Asymmetric DiMethyl-Histone H3-R2 antibody (STJ24011) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R2 antibody (Around R2) (STJ24011)

SKU:
STJ24011

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Asymmetric Histone H3-R2 (Around R2) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:100
ChIP-seq 1:20-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around R2
Immunogen: A synthetic asymmetric dimethylated peptide around R2 of human histone H3 (NP_003520.1).
Immunogen Sequence: MARTK
Background Enables sequence-specific DNA binding activity. Predicted to be involved in cell differentiation and regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including animal organ development; enteric nervous system development; and regulation of T-helper cell differentiation. Predicted to be located in nucleus. Predicted to be part of chromatin.

Information sourced from Uniprot.org

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