• Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R26 antibody (STJ23963) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 150s.
  • Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R26 antibody (STJ23963). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R26 antibody (Around Arg26) (STJ23963)

SKU:
STJ23963

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Asymmetric Histone H3-R26 (Around Arg26) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around Arg26
Immunogen: A synthetic asymmetric dimethylated peptide around R26 of human histone H3 (NP_003520.1).
Immunogen Sequence: AARKS
Background This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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