• Western blot analysis of extracts of various cell lines, using Asymmetric DiMethyl-Histone H3-R17 antibody (STJ23965). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
  • Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R17 antibody (STJ23965).
  • Immunofluorescence analysis of 293T cells using Asymmetric DiMethyl-Histone H3-R17 antibody (STJ23965). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R17 antibody (STJ23965) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R17 antibody (2-22) (STJ23965)

SKU:
STJ23965

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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Asymmetric Histone H3-R17 (2-22) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 2-22
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-22 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARTKQTARKSTGGKAPRKQL A
Background Predicted to enable RNA binding activity. Predicted to be involved in fertilization; gamete generation; and piRNA metabolic process. Predicted to be located in mitochondrion; pi-body; and piP-body.

Information sourced from Uniprot.org

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