• Western blot analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody (STJ23994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (STJ23994).
  • Immunohistochemistry of paraffin-embedded human mammary cancer using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using DiMethyl-Histone H3-K79 antibody (STJ23994) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (STJ23994). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug DiMethyl-Histone H3-K79 antibody (STJ23994). Western blot was performed from the immunoprecipitate using DiMethyl-Histone H3-K79 antibody (STJ23994) at a dilition of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K79 antibody (STJ23994) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-K79 antibody (STJ23994)

SKU:
STJ23994

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Histone H3-K79 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
IP 1:500-1:1000
ChIP 1:500-1:1000
ChIP-seq 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic dimethylated peptide around K79 of human Histone H3 (NP_003520.1).
Immunogen Sequence: DFKTD
Background Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S5P family of ribosomal proteins. It is located in the cytoplasm. This gene shares sequence similarity with mouse LLRep3. It is co-transcribed with the small nucleolar RNA gene U64, which is located in its third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Information sourced from Uniprot.org

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