• Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K9 antibody (STJ23985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K9 antibody (STJ23985).
  • Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23985) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using DiMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23985) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using DiMethyl-Histone H3-K9 rabbit polyclonal antibody (STJ23985) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K9 antibody (STJ23985). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using DiMethyl-Histone H3-K9 antibody (STJ23985) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Di-Methyl-Histone H3-K9 antibody (2-19) (STJ23985)

SKU:
STJ23985

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Histone H3-K9 (2-19) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:50-1:200
ChIP-seq 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: 2-19
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 2-19 of human histone H3 (NP_003520.1).
Immunogen Sequence: ARTKQTARKSTGGKAPRK
Background This gene encodes an abundant protein present in the small intestinal epithelium. It is thought to participate in the uptake and/or intracellular metabolism of vitamin A. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. This protein may also modulate the supply of retinoic acid to the nuclei of endometrial cells during the menstrual cycle.

Information sourced from Uniprot.org

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