• Western blot analysis of extracts of NIH/3T3 cells, using Symmetric DiMethyl-Histone H3-R8 antibody (STJ23997) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Dot-blot analysis of all sorts of methylation peptides using Symmetric DiMethyl-Histone H3-R8 antibody (STJ23997).
  • Immunofluorescence analysis of 293T cells using Symmetric DiMethyl-Histone H3-R8 antibody (STJ23997). Blue: DAPI for nuclear staining.

Anti-Di-Methyl-Histone H3-R8 antibody (Around R8) (STJ23997)

SKU:
STJ23997

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-Symmetric Histone H3-R8 (Around R8) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:50-1:200
ChIP 1:20-1:50
ChIP-seq 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: Around R8
Immunogen: A synthetic symmetric dimethylated peptide around R8 of human histone H3 (NP_003520.1).
Immunogen Sequence: TARKS
Background Mammalian cytochrome c oxidase (COX) catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. In yeast, 2 related COX assembly genes, SCO1 and SCO2 (synthesis of cytochrome c oxidase) , enable subunits 1 and 2 to be incorporated into the holoprotein. This gene is the human homolog to the yeast SCO1 gene.

Information sourced from Uniprot.org

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