• Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody (STJ118453) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody (STJ118453) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody (STJ118453) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (STJ118453) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-Di-Methyl-DNMT3A K44me2-K44 antibody (STJ118453)

SKU:
STJ118453

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Di-Methyl-DNMT3A-K44 is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Dnmt3a
Gene ID: 13435
Uniprot ID: DNM3A_MOUSE
Immunogen: A synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
Immunogen Sequence: ARKVG
Tissue Specificity Isoform 1 is expressed ubiquitously at low levels. Expression of isoform 2 is restricted to tissues containing cells which are undergoing active de novo methylation, including spleen, testis and thymus.
Post Translational Modifications Auto-methylated at Cys-706: auto-methylation takes place in absence of DNA substrate and inactivates the DNA methyltransferase activity. Inactivation by auto-methylation may be used to inactivate unused DNA methyltransferases in the cell. Sumoylated.sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
Function Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-706 in absence of DNA.
Protein Name Dna
Cytosine-5-Methyltransferase 3a
Dnmt3a
Cysteine Methyltransferase Dnmt3a
Dna Methyltransferase Mmuiiia
Dna Mtase Mmuiiia
M.mmuiiia
Database Links Reactome: R-MMU-212300
Reactome: -MMU-3214858
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Accumulates In The Major Satellite Repeats At Pericentric Heterochromatin
Alternative Antibody Names Anti-Dna antibody
Anti-Cytosine-5-Methyltransferase 3a antibody
Anti-Dnmt3a antibody
Anti-Cysteine Methyltransferase Dnmt3a antibody
Anti-Dna Methyltransferase Mmuiiia antibody
Anti-Dna Mtase Mmuiiia antibody
Anti-M.mmuiiia antibody
Anti-Dnmt3a antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance