Immunocytochemistry Antibodies | St John's Laboratory

STJ73652 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing plasma membrane and endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Somatostatin proprotein antibody (58-70) (STJ73652)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/ICC

Reactivity :

Human/Mouse/Rat/Dog/Pig

$421.88

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Immunofluorescence analysis of HepG2 cells using Sorbitol Dehydrogenase Rabbit polyclonal antibody (STJ11104653) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SORD antibody (100-200) (STJ11104653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using Sorbitol Dehydrogenase Rabbit polyclonal antibody (STJ25655) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SORD antibody (145-300) (STJ25655)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from Rat heart, using SOX10 Rabbit pAb (STJ111355) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-SOX10 antibody (270-360) (STJ111355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of C6 cells using SOX10 Rabbit polyclonal antibody (STJ117294) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SOX10 antibody (270-360) (STJ117294)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using SOX10 Rabbit mAb (STJ11102345) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-SOX10 antibody (350-450) [S5MR] (STJ11102345)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of SH-SY5Y cells using SOX11 Rabbit polyclonal antibody (STJ119929) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SOX11 antibody (1-100) (STJ119929)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of HepG2, using SOX14 rabbit polyclonal antibody (STJ115629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SOX14 antibody (91-240) (STJ115629)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using SOX2 antibody (STJ11101655) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SOX2 antibody (1-100) [S5MR] (STJ11101655)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

$197.78 - $867.38

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Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g SOX2 antibody (STJ25661). Western blot was performed from the immunoprecipitate using SOX2 antibody (STJ25661) at a dilution of 1:500.

Anti-SOX2 antibody (1-140) (STJ25661)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using SOX2 antibody (STJ113816) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SOX2 antibody (1-317) (STJ113816)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of SOX2 in NCCIT cells using SOX2 antibody (green) , and DAPI (blue).

Anti-SOX2 antibody (1-68 aa) [R74-1A-2] (STJA0016073)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse

$357.08

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Western blot of NIH 3T3 cell lysate showing specific immunolabeling of the ~35 kDa SOX2 protein.

Anti-SOX2 antibody (C-Term) (STJA0003864)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Mouse/Human

$613.58

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Anti-SOX2 antibody (Full Length) [M551] (STJA0005589)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$431.33

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Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.

Anti-SOX2 antibody [2A10-F11-F12] (STJ99043)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/FC/ICC

Reactivity :

Mouse

$194.40 - $469.80

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Western blot analysis of various lysates using SOX30 Rabbit polyclonal antibody (STJ113314) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-SOX30 antibody (604-753) (STJ113314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of mouse brain, using SOX5 antibody (STJ29065) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SOX5 antibody (1-120) (STJ29065)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using SOX6 Rabbit polyclonal antibody (STJ11105533) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SOX6 antibody (1-74) (STJ11105533)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using SOX9 antibody (STJ11101779) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-SOX9 antibody (169-300) [S9MR] (STJ11101779)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of NIH/3T3 cells using SP1 Rabbit polyclonal antibody (STJ116866) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SP1 antibody (420-620) (STJ116866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ Hep G2 cells with 1 Mu g SP1 rabbit monoclonal antibody (STJ11101745) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of SP1 in representative gene loci (TINF2) , as shown in figure.

Anti-SP1 antibody (500-600) [S5MR] (STJ11101745)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq/CUT&Tag

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of HeLa cells using SPAG5 antibody (STJ116304). Blue: DAPI for nuclear staining.

Anti-SPAG5 antibody (1-300) (STJ116304)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded human testis using SPAM1 Rabbit polyclonal antibody (STJ25670) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPAM1 antibody (100-300) (STJ25670)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using SPARC Rabbit polyclonal antibody (STJ116705) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPARC antibody (18-303) (STJ116705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using Spata5 Rabbit pAb (STJ11106703) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Spata5 antibody (STJ11106703)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of MCF7 cells using SPDEF antibody (STJ116049).

Anti-SPDEF antibody (1-335) (STJ116049)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HepG2 cells using SPG7 rabbit polyclonal antibody (STJ112287) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPG7 antibody (1-250) (STJ112287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-SPHK1 antibody (C-Term) [M540] (STJA0005595)

Clonality :

Monoclonal

Host :

Rat

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

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Immunofluorescence analysis of MCF7 cells using SPHK2 Rabbit pAb (STJ11107102) at a dilution of 1:300 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPHK2 antibody (480-654 aa) (STJ11107102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-SPHK2 antibody (N-Term) (STJA0005599)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Human/Mouse/Rat

$496.13

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Immunofluorescence analysis of HeLa cells using SPINK1 antibody (STJ115507). Blue: DAPI for nuclear staining.

Anti-SPINK1 antibody (24-79) (STJ115507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using SPINT1 antibody (STJ28494). Blue: DAPI for nuclear staining.

Anti-SPINT1 antibody (234-513) (STJ28494)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HeLa cells using SPINT2 antibody (STJ28832). Blue: DAPI for nuclear staining.

Anti-SPINT2 antibody (28-197) (STJ28832)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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Western blot analysis of various lysates, using SPN rabbit polyclonal antibody (STJ28495) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC) :U-138 MG, SH-SY5YExposure time: 30s.

Anti-SPN antibody (276-400) (STJ28495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-SPP1 antibody (N-Term) [M574] (STJA0005500)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

$496.13

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Immunofluorescence analysis of paraffin-embedded mouse skin using SPRR1A Rabbit polyclonal antibody (STJ119627) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.

Anti-SPRR1A antibody (73-89) (STJ119627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using SPRR3 antibody (STJ113942) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SPRR3 antibody (1-70) (STJ113942)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of paraffin-embedded mouse lung using alpha 1 Spectrin Rabbit polyclonal antibody (STJ114233) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPTA1 antibody (940-1160) (STJ114233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of U-2 OS cells using Alpha Fodrin Rabbit polyclonal antibody (STJ114871) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SPTAN1 antibody (950-1130) (STJ114871)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot of rat hippocampal lysate showing specific immunolabeling of the ~240 kDa alpha II spectrin protein.

Anti-SPTAN1 antibody [3D7] (STJA0003570)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Bovine/Human/Mouse/Rat

$613.58

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Western blot analysis of HepG2, using SQLE antibody (STJ111151) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SQLE antibody (300-574) (STJ111151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of HepG2 cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ110069) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (1-440) (STJ110069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 100 Mu g extracts of HepG2 cells using 3 Mu g SQSTM1/p62 antibody (STJ113162). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113162) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 200 Mu g extracts of C6 cells using 3 Mu g SQSTM1/p62 antibody (STJ113746). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (STJ113746) at a dilution of 1:1000.

Anti-SQSTM1 antibody (1-440) (STJ113746)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Immunofluorescence analysis of U2OS cells using SQSTM1/p62 Rabbit polyclonal antibody (STJ11104488) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody (340-440) (STJ11104488)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Anti-SQSTM1 antibody (341-440) [S3MR] (STJ11101773)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of C2C12 using SQSTM1 Rabbit monoclonal antibody (STJ11104923) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-SQSTM1 antibody [S4923RM] (STJ11104923)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse

$174.83 - $775.58

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Western blot analysis of extracts of various cell lines, using Src antibody (STJ11101656) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SRC antibody (1-100) [S6MR] (STJ11101656)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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