• Western blot analysis of extracts of various cell lines, using MMP7 antibody (STJ24590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of Rat uterus, using MMP7 antibody (STJ24590) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using MMP7 rabbit polyclonal antibody (STJ24590) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of A-549 cells using MMP7 rabbit polyclonal antibody (STJ24590) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using MMP7 rabbit polyclonal antibody (STJ24590) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-MMP7 antibody (89-103) (STJ24590)

SKU:
STJ24590

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP7 (89-103) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP7
Gene ID: 4316
Uniprot ID: MMP7_HUMAN
Immunogen Region: 89-103
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 89-103 of human MMP7 (NP_002414.1).
Immunogen Sequence: VPDVAEYSLFPNSPK
Function Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
Protein Name Matrilysin
Matrin
Matrix Metalloproteinase-7
Mmp-7
Pump-1 Protease
Uterine Metalloproteinase
Database Links Reactome: R-HSA-1442490
Reactome: R-HSA-1474228
Reactome: R-HSA-1592389
Reactome: R-HSA-2022090
Reactome: R-HSA-9009391
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrilysin antibody
Anti-Matrin antibody
Anti-Matrix Metalloproteinase-7 antibody
Anti-Mmp-7 antibody
Anti-Pump-1 Protease antibody
Anti-Uterine Metalloproteinase antibody
Anti-MMP7 antibody
Anti-MPSL1 antibody
Anti-PUMP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance