• CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1) , as shown in figure.
  • Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Dot-blot analysis of all sorts of peptides using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at 1:1000 dilution.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse lung using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of 293F cells using 5 Mu g MonoMethyl-Histone H3-K18 antibody (STJ11103411). Western blot was performed from the immunoprecipitate using MonoMethyl-Histone H3-K18 antibody (STJ11103411) at a dilution of 1:1000.
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K18 antibody (STJ11103411) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Mono-Methyl-Histone H3-K18 antibody [S1MR] (STJ11103411)

SKU:
STJ11103411

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IP/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Mono-Methyl-Histone H3-K18 is suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:1000
WB 1:500-1:1000
IHC-P 1:50-1:200
IP 1:500-1:1000
IF/ICC 1:50-1:200
ChIP 1:50-1:200
CUT&Tag 10⁵ cells/1 Mu g
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic monomethylated peptide around K18 of human histone H3 (P68431).
Immunogen Sequence: APRKQ
Background This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability.

Information sourced from Uniprot.org

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