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Anti-MMP9 antibody (538-707) (STJ113539)

Immunofluorescence analysis of L929 cells using MMP9 antibody (STJ113539) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry analysis of paraffin-embedded mouse spleen using MMP9 antibody (STJ113539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using MMP9 antibody (STJ113539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using MMP9 antibody (STJ113539) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Western blot analysis of K-562, using MMP9 Rabbit polyclonal antibody (STJ113539) at 1:600 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MMP9 antibody (538-707) (STJ113539)

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STJ113539

£169.50 - £449.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-MMP9 (538-707) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	MMP9
Gene ID:	4318
Uniprot ID:	MMP9_HUMAN
Immunogen Region:	538-707
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 538-707 of human MMP9 (NP_004985.2).
Immunogen Sequence:	KYWRFSEGRGSRPQGPFLIA DKWPALPRKLDSVFEERLSK KLFFFSGRQVWVYTGASVLG PRRLDKLGLGADVAQVTGAL RSGRGKMLLFSGRRLWRFDV KAQMVDPRSASEVDRMFPGV PLDTHDVFQYREKAYFCQDR FYWRVSSRSELNQVDQVGYV TYDILQCPED

Tissue Specificity	Detected in neutrophils (at protein level). Produced by normal alveolar macrophages and granulocytes.
Post Translational Modifications	Processing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9. N- and O-glycosylated.
Function	Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-\|-Leu bond. Cleaves NINJ1 to generate the Secreted ninjurin-1 form. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Protein Name	Matrix Metalloproteinase-9 Mmp-9 92 Kda Gelatinase 92 Kda Type Iv Collagenase Gelatinase B Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9
Database Links	Reactome: R-HSA-1433557 Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389 Reactome: R-HSA-2022090 Reactome: R-HSA-3928665 Reactome: R-HSA-6785807 Reactome: R-HSA-6798695 Reactome: R-HSA-9009391
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative Antibody Names	Anti-Matrix Metalloproteinase-9 antibody Anti-Mmp-9 antibody Anti-92 Kda Gelatinase antibody Anti-92 Kda Type Iv Collagenase antibody Anti-Gelatinase B antibody Anti-Gelb Cleaved Into - 67 Kda Matrix Metalloproteinase-9 - 82 Kda Matrix Metalloproteinase-9 antibody Anti-MMP9 antibody Anti-CLG4B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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