• Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody (STJ112444) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using MMP16 antibody (STJ112444) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast using MMP16 antibody (STJ112444) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MMP16 antibody (STJ112444) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung cancer using MMP16 antibody (STJ112444) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MMP16 antibody (STJ112444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MMP16 antibody (290-565) (STJ112444)

SKU:
STJ112444

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP16 (290-565) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP16
Gene ID: 4325
Uniprot ID: MMP16_HUMAN
Immunogen Region: 290-565
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
Immunogen Sequence: YGPPDKIPPPTRPLPTVPPH RSIPPADPRKNDRPKPPRPP TGRPSYPGAKPNICDGNFNT LAILRREMFVFKDQWFWRVR NNRVMDGYPMQITYFWRGLP PSIDAVYENSDGNFVFFKGN KYWVFKDTTLQPGYPHDLIT LGSGIPPHGIDSAIWWEDVG KTYFFKGDRYWRYSEEMKTM DPGYPKPITVWKGIPESPQG AFVHKENGFTYFYKGKEYWK FNNQILKVEPGYPRSILKD
Tissue Specificity Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function Endopeptidase that degrades various components of the extracellular matrix, such as collagen type III and fibronectin. Activates progelatinase A. Involved in the matrix remodeling of blood vessels. Isoform short cleaves fibronectin and also collagen type III, but at lower rate. It has no effect on type I, II, IV and V collagen. However, upon interaction with CSPG4, it may be involved in degradation and invasion of type I collagen by melanoma cells.
Protein Name Matrix Metalloproteinase-16
Mmp-16
Mmp-X2
Membrane-Type Matrix Metalloproteinase 3
Mt-Mmp 3
Mtmmp3
Membrane-Type-3 Matrix Metalloproteinase
Mt3-Mmp
Mt3mmp
Database Links Reactome: R-HSA-1592389
Cellular Localisation Isoform Long: Cell Membrane
Single-Pass Type I Membrane Protein
Extracellular Side
Localized At The Cell Surface Of Melanoma Cells
Isoform Short: Secreted
Extracellular Space
Extracellular Matrix
Cell Surface
Alternative Antibody Names Anti-Matrix Metalloproteinase-16 antibody
Anti-Mmp-16 antibody
Anti-Mmp-X2 antibody
Anti-Membrane-Type Matrix Metalloproteinase 3 antibody
Anti-Mt-Mmp 3 antibody
Anti-Mtmmp3 antibody
Anti-Membrane-Type-3 Matrix Metalloproteinase antibody
Anti-Mt3-Mmp antibody
Anti-Mt3mmp antibody
Anti-MMP16 antibody
Anti-C8orf57 antibody
Anti-MMPX2 antibody

Information sourced from Uniprot.org

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